Syntenin, a PDZ protein that binds syndecan cytoplasmic domains
10.1073/pnas.94.25.13683
Crossref journal-article
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences (341)
Abstract

The syndecans are transmembrane proteoglycans that place structurally heterogeneous heparan sulfate chains at the cell surface and a highly conserved polypeptide in the cytoplasm. Their versatile heparan sulfate moieties support various processes of molecular recognition, signaling, and trafficking. Here we report the identification of a protein that binds to the cytoplasmic domains of the syndecans in yeast two-hybrid screens, surface plasmon resonance experiments, and ligand-overlay assays. This protein, syntenin, contains a tandem repeat of PDZ domains that reacts with the FYA C-terminal amino acid sequence of the syndecans. Recombinant enhanced green fluorescent protein (eGFP)–syntenin fusion proteins decorate the plasmamembrane and intracellular vesicles, where they colocalize and cosegregate with syndecans. Cells that overexpress eGFP–syntenin show numerous cell surface extensions, suggesting effects of syntenin on cytoskeleton–membrane organization. We propose that syntenin may function as an adaptor that couples syndecans to cytoskeletal proteins or cytosolic downstream signal-effectors.

Bibliography

Grootjans, J. J., Zimmermann, P., Reekmans, G., Smets, A., Degeest, G., Dürr, J., & David, G. (1997). Syntenin, a PDZ protein that binds syndecan cytoplasmic domains. Proceedings of the National Academy of Sciences, 94(25), 13683–13688.

Authors 7
  1. Johan J. Grootjans (first)
  2. Pascale Zimmermann (additional)
  3. Gunter Reekmans (additional)
  4. An Smets (additional)
  5. Gisèle Degeest (additional)
  6. Joachim Dürr (additional)
  7. Guido David (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited 3 years, 2 months ago (June 6, 2022, 7:28 p.m.)
Indexed 1 day ago (Aug. 29, 2025, 5:48 a.m.)
Issued 27 years, 8 months ago (Dec. 9, 1997)
Published 27 years, 8 months ago (Dec. 9, 1997)
Published Online 27 years, 8 months ago (Dec. 9, 1997)
Published Print 27 years, 8 months ago (Dec. 9, 1997)
Funders 0

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@article{Grootjans_1997, title={Syntenin, a PDZ protein that binds syndecan cytoplasmic domains}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.25.13683}, DOI={10.1073/pnas.94.25.13683}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Grootjans, Johan J. and Zimmermann, Pascale and Reekmans, Gunter and Smets, An and Degeest, Gisèle and Dürr, Joachim and David, Guido}, year={1997}, month=dec, pages={13683–13688} }