DOI: 10.1073/pnas.94.25.13458. Histones H3 and H4 are components of upstream activation factor required for the high-level transcription of yeast rDNA by RNA polymerase I

Histones H3 and H4 are components of upstream activation factor required for the high-level transcription of yeast rDNA by RNA polymerase I

10.1073/pnas.94.25.13458

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

RNA polymerase I (Pol I) transcription in the yeast Saccharomyces cerevisiae is greatly stimulated in vivo and in vitro by the multiprotein complex, upstream activation factor (UAF). UAF binds tightly to the upstream element of the rDNA promoter, such that once bound ( in vitro ), UAF does not readily exchange onto a competing template. Of the polypeptides previously identified in purified UAF, three are encoded by genes required for Pol I transcription in vivo : RRN5 , RRN9 , and RRN10 . Two others, p30 and p18, have remained uncharacterized. We report here that the N-terminal amino acid sequence, its mobility in gel electrophoresis, and the immunoreactivity of p18 shows that it is histone H3. In addition, histone H4 was found in UAF, and myc-tagged histone H4 could be used to affinity-purify UAF. Histones H2A and H2B were not detectable in UAF. These results suggest that histones H3 and H4 probably account for the strong binding of UAF to DNA and may offer a means by which general nuclear regulatory signals could be transmitted to Pol I.

Bibliography

Keener, J., Dodd, J. A., Lalo, D., & Nomura, M. (1997). Histones H3 and H4 are components of upstream activation factor required for the high-level transcription of yeast rDNA by RNA polymeraseâI. Proceedings of the National Academy of Sciences, 94(25), 13458â13462.

Authors 4

John Keener (first)
Jonathan A. Dodd (additional)
Dominique Lalo (additional)
Masayasu Nomura (additional)

References 32 Referenced 73

10.1073/pnas.88.9.3962
10.1073/pnas.88.16.7026
10.1101/gad.8.19.2349
10.1101/gad.10.7.887
10.1002/j.1460-2075.1996.tb00770.x
10.1093/nar/20.2.279
10.1093/nar/17.23.9661
10.1093/nar/19.19.5363
10.1074/jbc.271.35.21062
10.1128/MCB.16.11.6436
10.1101/gad.10.20.2551
K E van Holde Chromatin (Springer, New York, 1988). / Chromatin by van Holde K E (1988)
A Wolffe Chromatin: Structure and Function (Academic, London, 1992). / Chromatin: Structure and Function by Wolffe A (1992)
10.1146/annurev.bi.63.070194.001405
10.1038/355219a0
10.1016/S0092-8674(00)80073-2
10.1016/S0092-8674(00)81918-2
10.1128/mcb.11.8.4111-4120.1991
10.1093/genetics/122.1.19
10.1101/gad.2.2.150
10.1101/gad.10.10.1247
10.1016/0092-8674(95)90512-X
10.1016/S0092-8674(00)81326-4
10.1016/0092-8674(89)90790-3
10.1093/nar/21.10.2331
10.1016/S0092-8674(00)80211-1
10.1016/S0092-8674(00)81316-1
10.1016/0092-8674(76)90145-8
10.1073/pnas.88.15.6829
10.1073/pnas.90.22.10489
10.1038/38444
10.1038/380356a0

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:48 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:24 a.m.)
Issued	27 years, 8 months ago (Dec. 9, 1997)
Published	27 years, 8 months ago (Dec. 9, 1997)
Published Online	27 years, 8 months ago (Dec. 9, 1997)
Published Print	27 years, 8 months ago (Dec. 9, 1997)

Funders 0

None

BibTeX

@article{Keener_1997, title={Histones H3 and H4 are components of upstream activation factor required for the high-level transcription of yeast rDNA by RNA polymerase I}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.25.13458}, DOI={10.1073/pnas.94.25.13458}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Keener, John and Dodd, Jonathan A. and Lalo, Dominique and Nomura, Masayasu}, year={1997}, month=dec, pages={13458–13462} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        11
      ]
    ],
    "date-time": "2025-07-11T10:24:50Z",
    "timestamp": 1752229490371
  },
  "reference-count": 32,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "25",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1997,
        12,
        9
      ]
    ]
  },
  "abstract": "<jats:p>\n            RNA polymerase I (Pol I) transcription in the yeast\n            <jats:italic>Saccharomyces cerevisiae</jats:italic>\n            is greatly stimulated\n            <jats:italic>in vivo</jats:italic>\n            and\n            <jats:italic>in vitro</jats:italic>\n            by the multiprotein complex, upstream activation factor (UAF). UAF binds tightly to the upstream element of the rDNA promoter, such that once bound (\n            <jats:italic>in vitro</jats:italic>\n            ), UAF does not readily exchange onto a competing template. Of the polypeptides previously identified in purified UAF, three are encoded by genes required for Pol I transcription\n            <jats:italic>in vivo</jats:italic>\n            :\n            <jats:italic>RRN5</jats:italic>\n            ,\n            <jats:italic>RRN9</jats:italic>\n            , and\n            <jats:italic>RRN10</jats:italic>\n            . Two others, p30 and p18, have remained uncharacterized. We report here that the N-terminal amino acid sequence, its mobility in gel electrophoresis, and the immunoreactivity of p18 shows that it is histone H3. In addition, histone H4 was found in UAF, and myc-tagged histone H4 could be used to affinity-purify UAF. Histones H2A and H2B were not detectable in UAF. These results suggest that histones H3 and H4 probably account for the strong binding of UAF to DNA and may offer a means by which general nuclear regulatory signals could be transmitted to Pol I.\n          </jats:p>",
  "DOI": "10.1073/pnas.94.25.13458",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:31:39Z",
    "timestamp": 1027693899000
  },
  "page": "13458-13462",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 73,
  "title": "Histones H3 and H4 are components of upstream activation factor required for the high-level transcription of yeast rDNA by RNA polymerase\u2009I",
  "prefix": "10.1073",
  "volume": "94",
  "author": [
    {
      "given": "John",
      "family": "Keener",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Biological Chemistry, University of California, Irvine, CA 92697-1700"
        }
      ]
    },
    {
      "given": "Jonathan A.",
      "family": "Dodd",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biological Chemistry, University of California, Irvine, CA 92697-1700"
        }
      ]
    },
    {
      "given": "Dominique",
      "family": "Lalo",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biological Chemistry, University of California, Irvine, CA 92697-1700"
        }
      ]
    },
    {
      "given": "Masayasu",
      "family": "Nomura",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Biological Chemistry, University of California, Irvine, CA 92697-1700"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1997,
        12,
        9
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.88.9.3962"
    },
    {
      "key": "e_1_3_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.88.16.7026"
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.19.2349"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.10.7.887"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00770.x"
    },
    {
      "key": "e_1_3_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/20.2.279"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/17.23.9661"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/19.19.5363"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.35.21062"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.16.11.6436"
    },
    {
      "key": "e_1_3_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.10.20.2551"
    },
    {
      "key": "e_1_3_3_12_2",
      "volume-title": "Chromatin",
      "author": "van Holde K E",
      "year": "1988",
      "unstructured": "K E van Holde Chromatin (Springer, New York, 1988)."
    },
    {
      "key": "e_1_3_3_13_2",
      "volume-title": "Chromatin: Structure and Function",
      "author": "Wolffe A",
      "year": "1992",
      "unstructured": "A Wolffe Chromatin: Structure and Function (Academic, London, 1992)."
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.63.070194.001405"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/355219a0"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80073-2"
    },
    {
      "key": "e_1_3_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81918-2"
    },
    {
      "key": "e_1_3_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.11.8.4111-4120.1991"
    },
    {
      "key": "e_1_3_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/122.1.19"
    },
    {
      "key": "e_1_3_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.2.2.150"
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.10.10.1247"
    },
    {
      "key": "e_1_3_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90512-X"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81326-4"
    },
    {
      "key": "e_1_3_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90790-3"
    },
    {
      "key": "e_1_3_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/21.10.2331"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80211-1"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81316-1"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(76)90145-8"
    },
    {
      "key": "e_1_3_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.88.15.6829"
    },
    {
      "key": "e_1_3_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.22.10489"
    },
    {
      "key": "e_1_3_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/38444"
    },
    {
      "key": "e_1_3_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/380356a0"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.94.25.13458",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T18:48:00Z",
    "timestamp": 1649875680000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.94.25.13458"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1997,
        12,
        9
      ]
    ]
  },
  "references-count": 32,
  "journal-issue": {
    "issue": "25",
    "published-print": {
      "date-parts": [
        [
          1997,
          12,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.94.25.13458"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.94.25.13458",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1997,
        12,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "1997-10-16",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1997-12-09",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}