DOI: 10.1073/pnas.94.24.13134. Preassembly of interleukin 2 (IL-2) receptor subunits on resting Kit 225 K6 T cells and their modulation by IL-2, IL-7, and IL-15: A fluorescence resonance energy transfer study

Preassembly of interleukin 2 (IL-2) receptor subunits on resting Kit 225 K6 T cells and their modulation by IL-2, IL-7, and IL-15: A fluorescence resonance energy transfer study

10.1073/pnas.94.24.13134

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Assembly and mutual proximities of α, β, and γcsubunits of the interleukin 2 receptors (IL-2R) in plasma membranes of Kit 225 K6 T lymphoma cells were investigated by fluorescence resonance energy transfer (FRET) using fluorescein isothiocyanate- and Cy3-conjugated monoclonal antibodies (mAbs) that were directed against the IL-2Rα, IL-2Rβ, and γcsubunits of IL-2R. The cell-surface distribution of subunits was analyzed at the nanometer scale (2–10 nm) by FRET on a cell-by-cell basis. The cells were probed in resting phase and after coculture with saturating concentrations of IL-2, IL-7, and IL-15. FRET data from donor- and acceptor-labeled IL-2Rβ-α, γ-α, and γ-β pairs demonstrated close proximity of all subunits to each other in the plasma membrane of resting T cells. These mutual proximities do not appear to represent mAb-induced microaggregation, because FRET measurements with Fab fragments of the mAbs gave similar results. The relative proximities were meaningfully modulated by binding of IL-2, IL-7, and IL-15. Based on FRET analysis the topology of the three subunits at the surface of resting cells can be best described by a “triangular model” in the absence of added interleukins. IL-2 strengthens the bridges between the subunits, making the triangle more compact. IL-7 and IL-15 act in the opposite direction by opening the triangle possibly because they associate their private specific α receptors with the β and/or γcsubunits of the IL-2R complex. These data suggest that IL-2R subunits are already colocalized in resting T cells and do not require cytokine-induced redistribution. This colocalization is significantly modulated by binding of relevant interleukins in a cytokine-specific manner.

Bibliography

Damjanovich, S., Bene, L., MatkÃ³, J., Alileche, A., Goldman, C. K., Sharrow, S., & Waldmann, T. A. (1997). Preassembly of interleukin 2 (IL-2) receptor subunits on resting Kit 225 K6 T cells and their modulation by IL-2, IL-7, and IL-15: A fluorescence resonance energy transfer study. Proceedings of the National Academy of Sciences, 94(24), 13134â13139.

Authors 7

Sándor Damjanovich (first)
László Bene (additional)
János Matkó (additional)
Abdelkrim Alileche (additional)
Carolyn K. Goldman (additional)
Susan Sharrow (additional)
Thomas A. Waldmann (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:10 a.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 6:29 a.m.)
Issued	27 years, 9 months ago (Nov. 25, 1997)
Published	27 years, 9 months ago (Nov. 25, 1997)
Published Online	27 years, 9 months ago (Nov. 25, 1997)
Published Print	27 years, 9 months ago (Nov. 25, 1997)

Funders 0

None

BibTeX

@article{Damjanovich_1997, title={Preassembly of interleukin 2 (IL-2) receptor subunits on resting Kit 225 K6 T cells and their modulation by IL-2, IL-7, and IL-15: A fluorescence resonance energy transfer study}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.24.13134}, DOI={10.1073/pnas.94.24.13134}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Damjanovich, Sándor and Bene, László and Matkó, János and Alileche, Abdelkrim and Goldman, Carolyn K. and Sharrow, Susan and Waldmann, Thomas A.}, year={1997}, month=nov, pages={13134–13139} }

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