DOI: 10.1073/pnas.94.23.12303. Atomic structure of a thermostable subdomain of HIV-1 gp41

Atomic structure of a thermostable subdomain of HIV-1 gp41

10.1073/pnas.94.23.12303

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Infection by HIV-1 involves the fusion of viral and cellular membranes with subsequent transfer of viral genetic material into the cell. The HIV-1 envelope glycoprotein that mediates fusion consists of the surface subunit gp120 and the transmembrane subunit gp41. gp120 directs virion attachment to the cell–surface receptors, and gp41 then promotes viral–cell membrane fusion. A soluble, α-helical, trimeric complex within gp41 composed of N-terminal and C-terminal extraviral segments has been proposed to represent the core of the fusion-active conformation of the HIV-1 envelope. A thermostable subdomain denoted N34(L6)C28 can be formed by the N-34 and C-28 peptides connected by a flexible linker in place of the disulfide-bonded loop region. Three-dimensional structure of N34(L6)C28 reveals that three molecules fold into a six-stranded helical bundle. Three N-terminal helices within the bundle form a central, parallel, trimeric coiled coil, whereas three C-terminal helices pack in the reverse direction into three hydrophobic grooves on the surface of the N-terminal trimer. This thermostable subdomain displays the salient features of the core structure of the isolated gp41 subunit and thus provides a possible target for therapeutics designed selectively to block HIV-1 entry.

Bibliography

Tan, K., Liu, J., Wang, J., Shen, S., & Lu, M. (1997). Atomic structure of a thermostable subdomain of HIV-1âgp41. Proceedings of the National Academy of Sciences, 94(23), 12303â12308.

Authors 5

Kemin Tan (first)
Jin-huan Liu (additional)
Jia-huai Wang (additional)
Steven Shen (additional)
Min Lu (additional)

References 56 Referenced 458

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 2 months ago (June 6, 2022, 7:31 p.m.)
Indexed	4 months, 1 week ago (April 11, 2025, 5:26 a.m.)
Issued	27 years, 9 months ago (Nov. 11, 1997)
Published	27 years, 9 months ago (Nov. 11, 1997)
Published Online	27 years, 9 months ago (Nov. 11, 1997)
Published Print	27 years, 9 months ago (Nov. 11, 1997)

Funders 0

None

BibTeX

@article{Tan_1997, title={Atomic structure of a thermostable subdomain of HIV-1 gp41}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.23.12303}, DOI={10.1073/pnas.94.23.12303}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Tan, Kemin and Liu, Jin-huan and Wang, Jia-huai and Shen, Steven and Lu, Min}, year={1997}, month=nov, pages={12303–12308} }

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