DOI: 10.1073/pnas.94.23.12297. RNA-peptide fusions for the <i>in vitro</i> selection of peptides and proteins

RNA-peptide fusions for the in vitro selection of peptides and proteins

10.1073/pnas.94.23.12297

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Covalent fusions between an mRNA and the peptide or protein that it encodes can be generated by in vitro translation of synthetic mRNAs that carry puromycin, a peptidyl acceptor antibiotic, at their 3′ end. The stable linkage between the informational (nucleic acid) and functional (peptide) domains of the resulting joint molecules allows a specific mRNA to be enriched from a complex mixture of mRNAs based on the properties of its encoded peptide. Fusions between a synthetic mRNA and its encoded myc epitope peptide have been enriched from a pool of random sequence mRNA-peptide fusions by immunoprecipitation. Covalent RNA-peptide fusions should provide an additional route to the in vitro selection and directed evolution of proteins.

Bibliography

Roberts, R. W., & Szostak, J. W. (1997). RNA-peptide fusions for the in vitro selection of peptides andâproteins. Proceedings of the National Academy of Sciences, 94(23), 12297â12302.

Authors 2

Richard W. Roberts (first)
Jack W. Szostak (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:22 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 6, 2025, 9:48 a.m.)
Issued	27 years, 9 months ago (Nov. 11, 1997)
Published	27 years, 9 months ago (Nov. 11, 1997)
Published Online	27 years, 9 months ago (Nov. 11, 1997)
Published Print	27 years, 9 months ago (Nov. 11, 1997)

Funders 0

None

BibTeX

@article{Roberts_1997, title={RNA-peptide fusions for the in vitro selection of peptides and proteins}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.23.12297}, DOI={10.1073/pnas.94.23.12297}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Roberts, Richard W. and Szostak, Jack W.}, year={1997}, month=nov, pages={12297–12302} }

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