DOI: 10.1073/pnas.94.23.12280. The ribosome-in-pieces: Binding of elongation factor EF-G to oligoribonucleotides that mimic the sarcin/ricin and thiostrepton domains of 23S ribosomal RNA

The ribosome-in-pieces: Binding of elongation factor EF-G to oligoribonucleotides that mimic the sarcin/ricin and thiostrepton domains of 23S ribosomal RNA

10.1073/pnas.94.23.12280

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

An oligoribonucleotide (a 27-mer) that mimics the sarcin/ricin (S/R) domain of Escherichia coli 23S rRNA binds elongation factor EF-G; the K d is 6.9 μM, whereas for binding to ribosomes it is 0.7 μM. Binding saturates when EF-G and the S/R RNA are equimolar; at saturation 70% of the input RNA is in complexes with EF-G. Binding of EF-G to S/R RNA does not require GTP but is inhibited by GDP; the inhibition by GDP is overcome by GTP. The effects of mutations of the S/R domain nucleotides G2655, A2660, and G2661 suggest that EF-G recognizes the conformation of the RNA rather than the identity of the nucleotides. EF-G also binds to an oligoribonucleotide (an 84-mer) that has the thiostrepton region of 23S rRNA; however, EF-G binds independently to S/R and thiostrepton oligoribonucleotides.

Bibliography

Munishkin, A., & Wool, I. G. (1997). The ribosome-in-pieces: Binding of elongation factor EF-G to oligoribonucleotides that mimic the sarcin/ricin and thiostrepton domains of 23S ribosomalâRNA. Proceedings of the National Academy of Sciences, 94(23), 12280â12284.

Authors 2

Alexander Munishkin (first)
Ira G. Wool (additional)

References 27 Referenced 70

10.1016/S0021-9258(18)91792-8
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:20 p.m.)
Indexed	3 months, 2 weeks ago (May 20, 2025, 9:50 a.m.)
Issued	27 years, 9 months ago (Nov. 11, 1997)
Published	27 years, 9 months ago (Nov. 11, 1997)
Published Online	27 years, 9 months ago (Nov. 11, 1997)
Published Print	27 years, 9 months ago (Nov. 11, 1997)

Funders 0

None

BibTeX

@article{Munishkin_1997, title={The ribosome-in-pieces: Binding of elongation factor EF-G to oligoribonucleotides that mimic the sarcin/ricin and thiostrepton domains of 23S ribosomal RNA}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.23.12280}, DOI={10.1073/pnas.94.23.12280}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Munishkin, Alexander and Wool, Ira G.}, year={1997}, month=nov, pages={12280–12284} }

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