DOI: 10.1073/pnas.94.23.12268. Trajectory of DNA in the RNA polymerase II transcription preinitiation complex

Trajectory of DNA in the RNA polymerase II transcription preinitiation complex

10.1073/pnas.94.23.12268

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

By using site-specific protein-DNA photocrosslinking, we define the positions of TATA-binding protein, transcription factor IIB, transcription factor IIF, and subunits of RNA polymerase II (RNAPII) relative to promoter DNA within the human transcription preinitiation complex. The results indicate that the interface between the largest and second-largest subunits of RNAPII forms an extended, ≈240 Å channel that interacts with promoter DNA both upstream and downstream of the transcription start. By using electron microscopy, we show that RNAPII compacts promoter DNA by the equivalent of ≈50 bp. Together with the published structure of RNAPII, the results indicate that RNAPII wraps DNA around its surface and suggest a specific model for the trajectory of the wrapped DNA.

Bibliography

Kim, T.-K., Lagrange, T., Wang, Y.-H., Griffith, J. D., Reinberg, D., & Ebright, R. H. (1997). Trajectory of DNA in the RNA polymerase II transcription preinitiationâcomplex. Proceedings of the National Academy of Sciences, 94(23), 12268â12273.

Authors 6

Tae-Kyung Kim (first)
Thierry Lagrange (additional)
Yuh-Hwa Wang (additional)
Jack D. Griffith (additional)
Danny Reinberg (additional)
Richard H. Ebright (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:15 p.m.)
Indexed	1 year ago (Aug. 6, 2024, 10:14 p.m.)
Issued	27 years, 9 months ago (Nov. 11, 1997)
Published	27 years, 9 months ago (Nov. 11, 1997)
Published Online	27 years, 9 months ago (Nov. 11, 1997)
Published Print	27 years, 9 months ago (Nov. 11, 1997)

Funders 0

None

BibTeX

@article{Kim_1997, title={Trajectory of DNA in the RNA polymerase II transcription preinitiation complex}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.23.12268}, DOI={10.1073/pnas.94.23.12268}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kim, Tae-Kyung and Lagrange, Thierry and Wang, Yuh-Hwa and Griffith, Jack D. and Reinberg, Danny and Ebright, Richard H.}, year={1997}, month=nov, pages={12268–12273} }

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}