DOI: 10.1073/pnas.94.22.11935. Mechanical separation of the complementary strands of DNA

Mechanical separation of the complementary strands of DNA

10.1073/pnas.94.22.11935

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage λ DNA. The 3′ and 5′ extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10–15 pN. The separation force signal is shown to be related to the local GC vs. AT content along the molecule. Variations of this content on a typical scale of 100–500 bases are presently detected.

Bibliography

Essevaz-Roulet, B., Bockelmann, U., & Heslot, F. (1997). Mechanical separation of the complementary strands ofâDNA. Proceedings of the National Academy of Sciences, 94(22), 11935â11940.

Authors 3

B. Essevaz-Roulet (first)
U. Bockelmann (additional)
F. Heslot (additional)

References 39 Referenced 386

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:23 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 6, 2025, 8:18 a.m.)
Issued	27 years, 10 months ago (Oct. 28, 1997)
Published	27 years, 10 months ago (Oct. 28, 1997)
Published Online	27 years, 10 months ago (Oct. 28, 1997)
Published Print	27 years, 10 months ago (Oct. 28, 1997)

Funders 0

None

BibTeX

@article{Essevaz_Roulet_1997, title={Mechanical separation of the complementary strands of DNA}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.22.11935}, DOI={10.1073/pnas.94.22.11935}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Essevaz-Roulet, B. and Bockelmann, U. and Heslot, F.}, year={1997}, month=oct, pages={11935–11940} }

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