DOI: 10.1073/pnas.94.22.11798. Prp43: An RNA helicase-like factor involved in spliceosome disassembly

Prp43: An RNA helicase-like factor involved in spliceosome disassembly

10.1073/pnas.94.22.11798

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The Saccharomyces cerevisiae genes PRP2 , PRP16 , and PRP22 encode pre-mRNA splicing factors that belong to the highly conserved “DEAH” family of putative RNA helicases. We previously identified two additional members of this family, JA1 and JA2 . To investigate its biological function, we cloned the JA1 gene and generated alleles carrying mutations identical to those found in highly conserved regions of other members of the DEAH family. A ja1 allele carrying a mutation identical to that in the temperature-sensitive (ts) prp22–1 gene conferred ts phenotype when integrated into the genome of a wild-type strain by gene replacement. Northern analysis of RNA obtained from the ts strain shifted to a nonpermissive temperature revealed accumulation of unspliced pre-mRNAs and excised intron lariats. Furthermore, analysis of splicing complexes showed that intron lariats accumulated in spliceosomes. The results presented indicate that JA1 encodes a p re-m R NA p rocessing factor (Prp) involved in disassembly of spliceosomes after the release of mature mRNA. We have therefore renamed this gene PRP43 .

Bibliography

Arenas, J. E., & Abelson, J. N. (1997). Prp43: An RNA helicase-like factor involved in spliceosomeâdisassembly. Proceedings of the National Academy of Sciences, 94(22), 11798â11802.

Authors 2

Jaime E. Arenas (first)
John N. Abelson (additional)

References 24 Referenced 192

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:42 a.m.)
Deposited	3 years, 2 months ago (June 6, 2022, 5:31 p.m.)
Indexed	1 month ago (July 24, 2025, 6:59 a.m.)
Issued	27 years, 9 months ago (Oct. 28, 1997)
Published	27 years, 9 months ago (Oct. 28, 1997)
Published Online	27 years, 9 months ago (Oct. 28, 1997)
Published Print	27 years, 9 months ago (Oct. 28, 1997)

Funders 0

None

BibTeX

@article{Arenas_1997, title={Prp43: An RNA helicase-like factor involved in spliceosome disassembly}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.22.11798}, DOI={10.1073/pnas.94.22.11798}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Arenas, Jaime E. and Abelson, John N.}, year={1997}, month=oct, pages={11798–11802} }

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