DOI: 10.1073/pnas.94.21.11520. Direct evidence to support the role of HLA-G in protecting the fetus from maternal uterine natural killer cytolysis

Direct evidence to support the role of HLA-G in protecting the fetus from maternal uterine natural killer cytolysis

10.1073/pnas.94.21.11520

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

HLA-G is a nonclassical major histocompatibility complex class I molecule selectively expressed on cytotrophoblasts at the feto–maternal interface, where it may play an important role in maternal tolerance of the fetus. We provide direct evidence under physiological conditions that supports the role of HLA-G in protecting cytotrophoblasts against natural killer (NK) cytolysis in 6 semiallogenic combinations of maternal uterine NK cells and their own trophoblast counterparts, as well as in 20 allogenic combinations of maternal uterine NK cells and trophoblasts from different mothers. We show that, in all cases studied, this HLA-G-mediated protection was abolished by treatment of cytotrophoblasts with an HLA-G-specific mAb. The HLA class I-negative K562 cell line transfected with the predominant HLA-G1 isoform results in similar protection and abolition from maternal uterine NK lysis. Because maternal uterine NK cells express killer inhibitory receptors for HLA-G, we conclude that their interactions contribute to the survival of the fetal semiallograft by confering immunological tolerance to its tissues.

Bibliography

Rouas-Freiss, N., GonÃ§alves, R. M.-B., Menier, C., Dausset, J., & Carosella, E. D. (1997). Direct evidence to support the role of HLA-G in protecting the fetus from maternal uterine natural killerâcytolysis. Proceedings of the National Academy of Sciences, 94(21), 11520â11525.

Authors 5

Nathalie Rouas-Freiss (first)
Rachel Marchal-Bras Gonçalves (additional)
Catherine Menier (additional)
Jean Dausset (additional)
Edgardo D. Carosella (additional)

References 25 Referenced 550

10.1126/science.2326636
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J J Perez-Villar, I Melero, F Navarro, M Carretero, T Bellon, M Llano, M Colonna, D E Geraghty, M Lopez-Botet J Immunol 158, 5736–5743 (1997). (10.4049/jimmunol.158.12.5736) / J Immunol by Perez-Villar J J (1997)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:08 a.m.)
Indexed	3 hours, 36 minutes ago (Aug. 30, 2025, 12:58 p.m.)
Issued	27 years, 10 months ago (Oct. 14, 1997)
Published	27 years, 10 months ago (Oct. 14, 1997)
Published Online	27 years, 10 months ago (Oct. 14, 1997)
Published Print	27 years, 10 months ago (Oct. 14, 1997)

Funders 0

None

BibTeX

@article{Rouas_Freiss_1997, title={Direct evidence to support the role of HLA-G in protecting the fetus from maternal uterine natural killer cytolysis}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.21.11520}, DOI={10.1073/pnas.94.21.11520}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Rouas-Freiss, Nathalie and Gonçalves, Rachel Marchal-Bras and Menier, Catherine and Dausset, Jean and Carosella, Edgardo D.}, year={1997}, month=oct, pages={11520–11525} }

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