DOI: 10.1073/pnas.94.21.11456. Functional association between promoter structure and transcript alternative splicing

Functional association between promoter structure and transcript alternative splicing

10.1073/pnas.94.21.11456

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

It has been assumed that constitutive and regulated splicing of RNA polymerase II transcripts depends exclusively on signals present in the RNA molecule. Here we show that changes in promoter structure strongly affect splice site selection. We investigated the splicing of the ED I exon, which encodes a facultative type III repeat of fibronectin, whose inclusion is regulated during development and in proliferative processes. We used an alternative splicing assay combined with promoter swapping to demonstrate that the extent of ED I splicing is dependent on the promoter structure from which the transcript originated and that this regulation is independent of the promoter strength. Thus, these results provide the first evidence for coupling between alternative splicing and promoter-specific transcription, which agrees with recent cytological and biochemical evidence of coordination between splicing and transcription.

Bibliography

Cramer, P., Pesce, C. G., Baralle, F. E., & Kornblihtt, A. R. (1997). Functional association between promoter structure and transcript alternativeâsplicing. Proceedings of the National Academy of Sciences, 94(21), 11456â11460.

Authors 4

Paula Cramer (first)
C. Gustavo Pesce (additional)
Francisco E. Baralle (additional)
Alberto R. Kornblihtt (additional)

References 29 Referenced 276

10.1016/0962-8924(93)90105-A
10.1126/science.8446901
10.1038/387523a0
10.1002/j.1460-2075.1984.tb01787.x
10.1073/pnas.84.20.7179
10.1096/fasebj.10.2.8641558
10.1093/nar/15.19.7725
10.1093/nar/22.6.1018
10.1101/gad.7.12a.2405
10.1002/j.1460-2075.1987.tb02570.x
10.1074/jbc.271.36.22271
10.1016/0003-2697(87)90021-2
10.1016/S0021-9258(18)42342-3
10.1016/0168-9525(96)10037-8
10.1101/gad.9.10.1278
10.1016/0092-8674(88)90202-4
10.1128/jvi.63.7.3026-3033.1989
10.1146/annurev.cb.08.110192.001025
S S Sisodia, B Sollner-Webb, D W Cleveland Mol Cell Biol 7, 3601–3612 (1987). / Mol Cell Biol by Sisodia S S (1987)
10.1093/nar/24.23.4649
10.1073/pnas.93.14.6975
10.1038/385357a0
10.1016/0968-0004(93)90016-G
10.1002/j.1460-2075.1995.tb07360.x
10.1073/pnas.92.11.5174
10.1002/j.1460-2075.1988.tb03188.x
10.1002/j.1460-2075.1994.tb06367.x
10.1126/science.8085156
10.1126/science.3043662

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:20 p.m.)
Indexed	2 weeks, 1 day ago (Aug. 6, 2025, 9:10 a.m.)
Issued	27 years, 10 months ago (Oct. 14, 1997)
Published	27 years, 10 months ago (Oct. 14, 1997)
Published Online	27 years, 10 months ago (Oct. 14, 1997)
Published Print	27 years, 10 months ago (Oct. 14, 1997)

Funders 0

None

BibTeX

@article{Cramer_1997, title={Functional association between promoter structure and transcript alternative splicing}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.21.11456}, DOI={10.1073/pnas.94.21.11456}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Cramer, Paula and Pesce, C. Gustavo and Baralle, Francisco E. and Kornblihtt, Alberto R.}, year={1997}, month=oct, pages={11456–11460} }

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