DOI: 10.1073/pnas.94.21.11201. Converting a transmembrane receptor to a soluble receptor: Recognition domain to effector domain signaling after excision of the transmembrane domain

Converting a transmembrane receptor to a soluble receptor: Recognition domain to effector domain signaling after excision of the transmembrane domain

10.1073/pnas.94.21.11201

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The bacterial aspartate receptor was reconstructed to eliminate the transmembrane domain, thus connecting the recognition domain directly to the effector domain. The resulting soluble receptor folded correctly and was no longer an integral membrane protein. Upon aspartate binding, this soluble receptor was stabilized to a similar extent as that of the native receptor. Of interest, this soluble receptor retained the ability to signal from the recognition to the effector domain. This result defines more clearly the role of the membrane and transmembrane domains in signal transduction and suggests that some ligand-induced motions in receptor proteins do not require the membrane or transmembrane domain for information transmission.

Bibliography

Ottemann, K. M., & Koshland, D. E. (1997). Converting a transmembrane receptor to a soluble receptor: Recognition domain to effector domain signaling after excision of the transmembraneâdomain. Proceedings of the National Academy of Sciences, 94(21), 11201â11204.

Authors 2

Karen M. Ottemann (first)
Daniel E. Koshland (additional)

References 46 Referenced 8

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:07 a.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 11:02 p.m.)
Issued	27 years, 10 months ago (Oct. 14, 1997)
Published	27 years, 10 months ago (Oct. 14, 1997)
Published Online	27 years, 10 months ago (Oct. 14, 1997)
Published Print	27 years, 10 months ago (Oct. 14, 1997)

Funders 0

None

BibTeX

@article{Ottemann_1997, title={Converting a transmembrane receptor to a soluble receptor: Recognition domain to effector domain signaling after excision of the transmembrane domain}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.21.11201}, DOI={10.1073/pnas.94.21.11201}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ottemann, Karen M. and Koshland, Daniel E.}, year={1997}, month=oct, pages={11201–11204} }

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