DOI: 10.1073/pnas.94.20.10768. Mitochondrial DNA rearrangements of <i>Podospora anserina</i> are under the control of the nuclear gene grisea

Mitochondrial DNA rearrangements of Podospora anserina are under the control of the nuclear gene grisea

10.1073/pnas.94.20.10768

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Podospora anserina is a filamentous fungus with a limited life span. Life span is controlled by nuclear and extranuclear genetic traits. Herein we report the nature of four alterations in the nuclear gene grisea that lead to an altered morphology, a defect in the formation of female gametangia, and an increased life span. Three sequence changes are located in the 5′ upstream region of the grisea ORF. One mutation is a G → A transition at the 5′ splice site of the single intron of the gene, leading to a RNA splicing defect. This loss-of-function affects the amplification of the first intron of the mitochondrial cytochrome c oxidase subunit I gene ( COI ) and the specific mitochondrial DNA rearrangements that occur during senescence of wild-type strains. Our results indicate that the nuclear gene grisea is part of a molecular machinery involved in the control of mitochondrial DNA reorganizations. These DNA instabilities accelerate but are not a prerequisite for the aging of P. anserina cultures.

Bibliography

Borghouts, C., Kimpel, E., & Osiewacz, H. D. (1997). Mitochondrial DNA rearrangements of Podospora anserina are under the control of the nuclear geneâgrisea. Proceedings of the National Academy of Sciences, 94(20), 10768â10773.

Authors 3

Corina Borghouts (first)
Erik Kimpel (additional)
Heinz D. Osiewacz (additional)

References 50 Referenced 68

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:58 p.m.)
Indexed	3 months ago (May 24, 2025, 4:03 a.m.)
Issued	27 years, 11 months ago (Sept. 30, 1997)
Published	27 years, 11 months ago (Sept. 30, 1997)
Published Online	27 years, 11 months ago (Sept. 30, 1997)
Published Print	27 years, 11 months ago (Sept. 30, 1997)

Funders 0

None

BibTeX

@article{Borghouts_1997, title={Mitochondrial DNA rearrangements of Podospora anserina are under the control of the nuclear gene grisea}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.20.10768}, DOI={10.1073/pnas.94.20.10768}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Borghouts, Corina and Kimpel, Erik and Osiewacz, Heinz D.}, year={1997}, month=sep, pages={10768–10773} }

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