DOI: 10.1073/pnas.94.20.10541. The Aer protein and the serine chemoreceptor Tsr independently sense intracellular energy levels and transduce oxygen, redox, and energy signals for <i>Escherichia coli</i> behavior

The Aer protein and the serine chemoreceptor Tsr independently sense intracellular energy levels and transduce oxygen, redox, and energy signals for Escherichia coli behavior

10.1073/pnas.94.20.10541

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We identified a protein, Aer, as a signal transducer that senses intracellular energy levels rather than the external environment and that transduces signals for aerotaxis (taxis to oxygen) and other energy-dependent behavioral responses in Escherichia coli . Domains in Aer are similar to the signaling domain in chemotaxis receptors and the putative oxygen-sensing domain of some transcriptional activators. A putative FAD-binding site in the N-terminal domain of Aer shares a consensus sequence with the NifL, Bat, and Wc-1 signal-transducing proteins that regulate gene expression in response to redox changes, oxygen, and blue light, respectively. A double mutant deficient in aer and tsr , which codes for the serine chemoreceptor, was negative for aerotaxis, redox taxis, and glycerol taxis, each of which requires the proton motive force and/or electron transport system for signaling. We propose that Aer and Tsr sense the proton motive force or cellular redox state and thereby integrate diverse signals that guide E. coli to environments where maximal energy is available for growth.

Authors 7

Anuradha Rebbapragada (first)
Mark S. Johnson (additional)
Gordon P. Harding (additional)
Anthony J. Zuccarelli (additional)
Hansel M. Fletcher (additional)
Igor B. Zhulin (additional)
Barry L. Taylor (additional)

References 51 Referenced 232

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:58 p.m.)
Indexed	2 weeks, 3 days ago (Aug. 6, 2025, 9:53 a.m.)
Issued	27 years, 10 months ago (Sept. 30, 1997)
Published	27 years, 10 months ago (Sept. 30, 1997)
Published Online	27 years, 10 months ago (Sept. 30, 1997)
Published Print	27 years, 10 months ago (Sept. 30, 1997)

Funders 0

None

BibTeX

@article{Rebbapragada_1997, title={The Aer protein and the serine chemoreceptor Tsr independently sense intracellular energy levels and transduce oxygen, redox, and energy signals for Escherichia coli behavior}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.20.10541}, DOI={10.1073/pnas.94.20.10541}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Rebbapragada, Anuradha and Johnson, Mark S. and Harding, Gordon P. and Zuccarelli, Anthony J. and Fletcher, Hansel M. and Zhulin, Igor B. and Taylor, Barry L.}, year={1997}, month=sep, pages={10541–10546} }

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