DOI: 10.1073/pnas.94.2.575. Characterization of the chicken β-globin insulator

Characterization of the chicken β-globin insulator

10.1073/pnas.94.2.575

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Insulators, first identified in Drosophila , are DNA sequence elements that shield a promoter from nearby regulatory elements. We have previously reported that a DNA sequence at the 5′ end of the chicken β-globin locus can function as an insulator. It is capable of shielding a reporter gene from the activating effects of a nearby mouse β-globin locus control region element in the human erythroleukemic cell line K562. In this report, we show that most of the insulating activity lies in a 250-bp CpG island (core element), which contains the constitutive DNase I-hypersensitive site (5′HS4). DNA binding assays with the core sequence reveal a complex protein binding pattern. The insulating activity of the core element is multiplied when tandem copies are used. Although CpG islands are often associated with promoters of housekeeping genes, we find little evidence that the core element is a promoter. Furthermore, the insulator differs from a promoter in its ability to block the locus control region effect directionally.

Bibliography

Chung, J. H., Bell, A. C., & Felsenfeld, G. (1997). Characterization of the chickenâÎ²-globinâinsulator. Proceedings of the National Academy of Sciences, 94(2), 575â580.

Authors 3

Jay H. Chung (first)
Adam C. Bell (additional)
Gary Felsenfeld (additional)

References 21 Referenced 301

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:15 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:52 p.m.)
Issued	28 years, 7 months ago (Jan. 21, 1997)
Published	28 years, 7 months ago (Jan. 21, 1997)
Published Online	28 years, 7 months ago (Jan. 21, 1997)
Published Print	28 years, 7 months ago (Jan. 21, 1997)

Funders 0

None

BibTeX

@article{Chung_1997, title={Characterization of the chicken β-globin insulator}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.2.575}, DOI={10.1073/pnas.94.2.575}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Chung, Jay H. and Bell, Adam C. and Felsenfeld, Gary}, year={1997}, month=jan, pages={575–580} }

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