DOI: 10.1073/pnas.94.2.428. RGS4 and GAIP are GTPase-activating proteins for G <sub>qα</sub> and block activation of phospholipase Cβ by γ-thio-GTP-G <sub>qα</sub>

RGS4 and GAIP are GTPase-activating proteins for G qα and block activation of phospholipase Cβ by γ-thio-GTP-G qα

10.1073/pnas.94.2.428

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

RGS proteins constitute a newly appreciated and large group of negative r egulators of G protein s ignaling. Four members of the RGS family act as GTPase-activating proteins (GAPs) with apparent specificity for members of the G iα subfamily of G protein subunits. We demonstrate here that two RGS proteins, RGS4 and GAIP, also act as GAPs for G qα , the G α protein responsible for activation of phospholipase Cβ. Furthermore, these RGS proteins block activation of phospholipase Cβ by guanosine 5′-(3- O -thio)triphosphate-G qα . GAP activity does not explain this effect, which apparently results from occlusion of the binding site on G α for effector. Inhibitory effects of RGS proteins on G protein-mediated signaling pathways can be demonstrated by simple mixture of RGS4 or GAIP with plasma membranes.

Bibliography

Hepler, J. R., Berman, D. M., Gilman, A. G., & Kozasa, T. (1997). RGS4 and GAIP are GTPase-activating proteins for G qÎ± and block activation of phospholipase CÎ² byâÎ³-thio-GTP-G qÎ±. Proceedings of the National Academy of Sciences, 94(2), 428â432.

Authors 4

John R. Hepler (first)
David M. Berman (additional)
Alfred G. Gilman (additional)
Tohru Kozasa (additional)

References 39 Referenced 316

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:12 p.m.)
Indexed	4 months, 3 weeks ago (April 5, 2025, 4:02 p.m.)
Issued	28 years, 7 months ago (Jan. 21, 1997)
Published	28 years, 7 months ago (Jan. 21, 1997)
Published Online	28 years, 7 months ago (Jan. 21, 1997)
Published Print	28 years, 7 months ago (Jan. 21, 1997)

Funders 0

None

BibTeX

@article{Hepler_1997, title={RGS4 and GAIP are GTPase-activating proteins for G qα and block activation of phospholipase Cβ by γ-thio-GTP-G qα}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.2.428}, DOI={10.1073/pnas.94.2.428}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hepler, John R. and Berman, David M. and Gilman, Alfred G. and Kozasa, Tohru}, year={1997}, month=jan, pages={428–432} }

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