DOI: 10.1073/pnas.94.17.9114. A micromachined device provides a new bend on fibroblast traction forces

A micromachined device provides a new bend on fibroblast traction forces

10.1073/pnas.94.17.9114

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have measured the traction forces generated by fibroblasts using a novel micromachined device that is capable of determining the subcellular forces generated by individual adhesive contacts. The front of migrating fibroblasts produced intermittent rearward forces whereas the tail produced larger forward directed forces. None of the forces were steady; they all had periodic fluctuations. The transition between forward and rearward traction forces occurred at the nucleus, not at the rear of the cell or the border between the endoplasm and the ectoplasm. We propose that the coupling of lamella extensions to fluctuating rearward tractions in front of the nuclear region move the front of a fibroblast forward, while force-facilitated release of rear adhesive contacts and anterior-directed tractions allow the region behind the nucleus to advance.

Bibliography

Galbraith, C. G., & Sheetz, M. P. (1997). A micromachined device provides a new bend on fibroblast tractionâforces. Proceedings of the National Academy of Sciences, 94(17), 9114â9118.

Authors 2

Catherine G. Galbraith (first)
Michael P. Sheetz (additional)

References 23 Referenced 318

10.1083/jcb.101.2.597
10.1016/0014-4827(70)90570-7
10.1083/jcb.127.6.1957
10.1002/cm.970310306
10.1038/290249a0
10.1006/scel.1994.1018
C Galbraith, M Sheetz Optic, Electronic, and High Precision Measuring Techniques in Cell Biology, ed G Isenberg (Springer, Heidelberg, , in press. (1997). / Optic, Electronic, and High Precision Measuring Techniques in Cell Biology by Galbraith C (1997)
10.1083/jcb.107.2.665
10.1016/0092-8674(78)90053-3
A Harris, P Wild, D Stopak Science 208, 117–118 (1980). / Science by Harris A (1980)
10.1083/jcb.90.1.222
10.1083/jcb.131.4.989
10.1083/jcb.111.6.2513
10.1016/0014-4827(72)90084-5
10.1038/222641a0
10.1016/0014-4827(70)90646-4
10.1083/jcb.90.1.187
10.1016/S0006-3495(91)82027-6
10.1083/jcb.123.4.977
10.1083/jcb.119.5.1347
10.1126/science.3277283
10.1016/S0092-8674(00)81281-7
10.1083/jcb.136.6.1287

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:28 p.m.)
Indexed	1 week, 1 day ago (Aug. 23, 2025, 9:52 p.m.)
Issued	28 years ago (Aug. 19, 1997)
Published	28 years ago (Aug. 19, 1997)
Published Online	28 years ago (Aug. 19, 1997)
Published Print	28 years ago (Aug. 19, 1997)

Funders 0

None

BibTeX

@article{Galbraith_1997, title={A micromachined device provides a new bend on fibroblast traction forces}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.17.9114}, DOI={10.1073/pnas.94.17.9114}, number={17}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Galbraith, Catherine G. and Sheetz, Michael P.}, year={1997}, month=aug, pages={9114–9118} }

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