DOI: 10.1073/pnas.94.16.8812. Transcription initiation is controlled by three core promoter elements in the <i>hgl5</i> gene of the protozoan parasite <i>Entamoeba histolytica</i>

Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica

10.1073/pnas.94.16.8812

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Entamoeba histolytica is a single cell eukaryote that is the etiologic agent of amoebic colitis. Core promoter elements of E. histolytica protein encoding genes include a TATA-like sequence (GTATTTAAA G / C ) at −30, a novel element designated GAAC (GAACT) that has a variable location between TATA and the site of transcription initiation, and a putative initiator (Inr) element (AAAAATTCA) overlying the site of transcription initiation. The presence of three separate conserved sequences in a eukaryotic core promoter is unprecedented and prompted examination of their roles in regulating transcription initiation. Alterations of all three regions in the hgl5 gene decreased reporter gene activity with the greatest effect seen by mutation of the GAAC element. Positional analysis of the TATA box demonstrated that transcription initiated consistently 30–31 bases downstream of the TATA region. Mutation of either the TATA or GAAC elements resulted in the appearance of new transcription start sites upstream of +1 in the promoter of the hgl5 gene. Mutation of the Inr element resulted in no change in the site of transcription initiation; however, in the presence of a mutated TATA and GAAC regions, the Inr element controlled the site of transcription initiation. We conclude that all three elements play a role in determining the site of transcription initiation. The variable position of the GAAC element relative to the site of transcription initiation, and the multiple transcription initiations that resulted from its mutation, indicate that the GAAC element has an important and apparently novel role in transcriptional control in E. histolytica .

Bibliography

Singh, U., Rogers, J. B., Mann, B. J., & Petri, W. A. (1997). Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoebaâhistolytica. Proceedings of the National Academy of Sciences, 94(16), 8812â8817.

Authors 4

Upinder Singh (first)
Joshua B. Rogers (additional)
Barbara J. Mann (additional)
William A. Petri (additional)

References 29 Referenced 49

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M E Greenberg, T P Bender Current Protocols in Molecular Biology, eds F M Ausubel, R Brent, R E Kingston, D D Moore, J G Seidman, J A Smith, K Struhl (Wiley, New York), pp. 4.10.1–4.10.11 (1997). / Current Protocols in Molecular Biology by Greenberg M E (1997)
10.3181/00379727-203-43583
S T Smale Transcription: Mechanisms and Regulation, eds R C Conaway, J W Conaway (Raven, New York), pp. 63–81 (1994). / Transcription: Mechanisms and Regulation by Smale S T (1994)
10.1016/S0021-9258(18)48443-8
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10.1016/0042-6822(88)90657-5

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:46 p.m.)
Indexed	1 year, 1 month ago (July 25, 2024, 3:16 a.m.)
Issued	28 years, 1 month ago (Aug. 5, 1997)
Published	28 years, 1 month ago (Aug. 5, 1997)
Published Online	28 years, 1 month ago (Aug. 5, 1997)
Published Print	28 years, 1 month ago (Aug. 5, 1997)

Funders 0

None

BibTeX

@article{Singh_1997, title={Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.16.8812}, DOI={10.1073/pnas.94.16.8812}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Singh, Upinder and Rogers, Joshua B. and Mann, Barbara J. and Petri, William A.}, year={1997}, month=aug, pages={8812–8817} }

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