DOI: 10.1073/pnas.94.16.8521. <i>In vitro</i> selection of a 7-methyl-guanosine binding RNA that inhibits translation of capped mRNA molecules

In vitro selection of a 7-methyl-guanosine binding RNA that inhibits translation of capped mRNA molecules

10.1073/pnas.94.16.8521

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Using systematic evolution of ligands by exponential enrichment (SELEX), an RNA molecule was isolated that displays a 1,000-fold higher affinity for guanosine residues that carry an N-7 methyl group than for nonmethylated guanosine residues. The methylated guanosine residue closely resembles the 5′ terminal cap structure present on all eukaryotic mRNA molecules. The cap-binding RNA specifically inhibited the translation of capped but not uncapped mRNA molecules in cell-free lysates prepared from either human HeLa cells or from Saccharomyces cerevisiae . These findings indicate that the cap-binding RNA will also be useful in studies of other cap-dependent processes such as pre-mRNA splicing and nucleocytoplasmic mRNA transport.

Bibliography

Haller, A. A., & Sarnow, P. (1997). In vitro selection of a 7-methyl-guanosine binding RNA that inhibits translation of capped mRNAâmolecules. Proceedings of the National Academy of Sciences, 94(16), 8521â8526.

Authors 2

Aurelia A. Haller (first)
Peter Sarnow (additional)

References 34 Referenced 56

10.1016/0092-8674(76)90128-8
10.1128/mr.44.2.175-205.1980
10.1038/266235a0
10.1016/0092-8674(84)90268-X
10.1073/pnas.82.22.7590
10.1016/0092-8674(84)90049-7
10.1093/nar/15.4.1387
10.1101/gad.3.9.1472
10.1101/gad.10.13.1683
10.1073/pnas.84.15.5187
10.1016/0092-8674(90)90292-M
10.1083/jcb.118.6.1287
10.1038/376709a0
10.1073/pnas.76.9.4345
10.1126/science.275.5299.500
10.1016/S0092-8674(00)80268-8
J D Pata Mechanistic Studies of Poliovirus RNA-Dependent RNA Polymerase (Univ. of Colorado, Boulder, 1993). / Mechanistic Studies of Poliovirus RNA-Dependent RNA Polymerase by Pata J D (1993)
10.1101/gad.6.12b.2542
10.1128/mcb.14.11.7322-7330.1994
D G Macejak, S J Hambidge, L M Najita, P Sarnow EIF-4F-Independent Translation of Poliovirus RNA and Cellular mRNA Encoding Glucose-Regulated Protein 78/Immunoglobulin Heavy-Chain Binding Protein, eds M A Brinton, F X Heinz (Am. Soc. for Microbiol., Washington, DC), pp. 152–157 (1990). / EIF-4F-Independent Translation of Poliovirus RNA and Cellular mRNA Encoding Glucose-Regulated Protein 78/Immunoglobulin Heavy-Chain Binding Protein by Macejak D G (1990)
10.1128/jvi.67.6.2961-2971.1993
10.1128/MCB.16.7.3523
10.1128/mcb.7.2.725-737.1987
10.1101/gad.9.23.2997
10.1038/346818a0
10.1126/science.2200121
10.1038/364550a0
10.1126/science.7510417
10.1021/bi00297a001
10.1021/bi00473a002
10.1002/j.1460-2075.1996.tb01108.x
10.1002/j.1460-2075.1994.tb06699.x
10.1038/353090a0
10.1016/0092-8674(94)90530-4

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:52 p.m.)
Indexed	1 year, 2 months ago (June 8, 2024, 5:56 p.m.)
Issued	28 years ago (Aug. 5, 1997)
Published	28 years ago (Aug. 5, 1997)
Published Online	28 years ago (Aug. 5, 1997)
Published Print	28 years ago (Aug. 5, 1997)

Funders 0

None

BibTeX

@article{Haller_1997, title={In vitro selection of a 7-methyl-guanosine binding RNA that inhibits translation of capped mRNA molecules}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.16.8521}, DOI={10.1073/pnas.94.16.8521}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Haller, Aurelia A. and Sarnow, Peter}, year={1997}, month=aug, pages={8521–8526} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        6,
        8
      ]
    ],
    "date-time": "2024-06-08T21:56:40Z",
    "timestamp": 1717883800409
  },
  "reference-count": 34,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "16",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1997,
        8,
        5
      ]
    ]
  },
  "abstract": "<jats:p>\n            Using systematic evolution of ligands by exponential enrichment (SELEX), an RNA molecule was isolated that displays a 1,000-fold higher affinity for guanosine residues that carry an N-7 methyl group than for nonmethylated guanosine residues. The methylated guanosine residue closely resembles the 5\u2032 terminal cap structure present on all eukaryotic mRNA molecules. The cap-binding RNA specifically inhibited the translation of capped but not uncapped mRNA molecules in cell-free lysates prepared from either human HeLa cells or from\n            <jats:italic>Saccharomyces cerevisiae</jats:italic>\n            . These findings indicate that the cap-binding RNA will also be useful in studies of other cap-dependent processes such as pre-mRNA splicing and nucleocytoplasmic mRNA transport.\n          </jats:p>",
  "DOI": "10.1073/pnas.94.16.8521",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:31:44Z",
    "timestamp": 1027693904000
  },
  "page": "8521-8526",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 56,
  "title": "<i>In vitro</i>\n            selection of a 7-methyl-guanosine binding RNA that inhibits translation of capped mRNA\u2009molecules",
  "prefix": "10.1073",
  "volume": "94",
  "author": [
    {
      "given": "Aurelia A.",
      "family": "Haller",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5402; and Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, CO 80262"
        }
      ]
    },
    {
      "given": "Peter",
      "family": "Sarnow",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5402; and Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, CO 80262"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1997,
        8,
        5
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(76)90128-8"
    },
    {
      "key": "e_1_3_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mr.44.2.175-205.1980"
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/266235a0"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(84)90268-X"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.82.22.7590"
    },
    {
      "key": "e_1_3_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(84)90049-7"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/15.4.1387"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.3.9.1472"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.10.13.1683"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.84.15.5187"
    },
    {
      "key": "e_1_3_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90292-M"
    },
    {
      "key": "e_1_3_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.118.6.1287"
    },
    {
      "key": "e_1_3_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/376709a0"
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.76.9.4345"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.275.5299.500"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80268-8"
    },
    {
      "key": "e_1_3_3_17_2",
      "volume-title": "Mechanistic Studies of Poliovirus RNA-Dependent RNA Polymerase",
      "author": "Pata J D",
      "year": "1993",
      "unstructured": "J D Pata Mechanistic Studies of Poliovirus RNA-Dependent RNA Polymerase (Univ. of Colorado, Boulder, 1993)."
    },
    {
      "key": "e_1_3_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.12b.2542"
    },
    {
      "key": "e_1_3_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.14.11.7322-7330.1994"
    },
    {
      "key": "e_1_3_3_20_2",
      "first-page": "152",
      "volume-title": "EIF-4F-Independent Translation of Poliovirus RNA and Cellular mRNA Encoding Glucose-Regulated Protein 78/Immunoglobulin Heavy-Chain Binding Protein",
      "author": "Macejak D G",
      "year": "1990",
      "unstructured": "D G Macejak, S J Hambidge, L M Najita, P Sarnow EIF-4F-Independent Translation of Poliovirus RNA and Cellular mRNA Encoding Glucose-Regulated Protein 78/Immunoglobulin Heavy-Chain Binding Protein, eds M A Brinton, F X Heinz (Am. Soc. for Microbiol., Washington, DC), pp. 152\u2013157 (1990)."
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jvi.67.6.2961-2971.1993"
    },
    {
      "key": "e_1_3_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.16.7.3523"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.7.2.725-737.1987"
    },
    {
      "key": "e_1_3_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.9.23.2997"
    },
    {
      "key": "e_1_3_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/346818a0"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.2200121"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/364550a0"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7510417"
    },
    {
      "key": "e_1_3_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00297a001"
    },
    {
      "key": "e_1_3_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00473a002"
    },
    {
      "key": "e_1_3_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb01108.x"
    },
    {
      "key": "e_1_3_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1994.tb06699.x"
    },
    {
      "key": "e_1_3_3_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/353090a0"
    },
    {
      "key": "e_1_3_3_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90530-4"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.94.16.8521",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T18:52:18Z",
    "timestamp": 1649875938000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.94.16.8521"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1997,
        8,
        5
      ]
    ]
  },
  "references-count": 34,
  "journal-issue": {
    "issue": "16",
    "published-print": {
      "date-parts": [
        [
          1997,
          8,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.94.16.8521"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.94.16.8521",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1997,
        8,
        5
      ]
    ]
  },
  "assertion": [
    {
      "value": "1997-03-28",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1997-05-27",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1997-08-05",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}