DOI: 10.1073/pnas.94.15.8156. Growth arrest of Epstein–Barr virus immortalized B lymphocytes by adenovirus-delivered ribozymes

Growth arrest of Epstein–Barr virus immortalized B lymphocytes by adenovirus-delivered ribozymes

10.1073/pnas.94.15.8156

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Epstein–Barr virus (EBV) infection is associated with several human diseases that involve unrestricted proliferation of B lymphocytes. EBV nuclear antigen 1 (EBNA-1) is expressed in all EBV-infected cells and plays an essential role in persistence of the EBV genome. EBNA-1 has also been reported to have oncogenic potential. As an approach for treating EBV infections, we examined the capacity of EBNA-1 ribozymes delivered by recombinant adenoviruses to suppress EBNA-1 expression and to block virus-induced B cell proliferation. In contrast to primary B cells, EBV-transformed B lymphoblastoid cell lines expressed αv integrins, the adenovirus internalization receptors, and were also susceptible to adenovirus-mediated gene delivery. Adenovirus delivery of a specific ribozyme (RZ1) to lymphoblastoid cell lines, suppressed EBNA-1 mRNA and protein expression, significantly reduced the number of EBV genomes, and nearly abolished cell proliferation in low serum. Adenovirus delivery of RZ1 also prevented EBV infection of an established EBV-negative B cell line. These studies demonstrate the potential use of adenovirus-encoded ribozymes to treat EBV-induced lymphoproliferative disorders.

Bibliography

Huang, S., Stupack, D., Mathias, P., Wang, Y., & Nemerow, G. (1997). Growth arrest of EpsteinâBarr virus immortalized B lymphocytes by adenovirus-deliveredâribozymes. Proceedings of the National Academy of Sciences, 94(15), 8156â8161.

Authors 5

Shuang Huang (first)
Dwayne Stupack (additional)
Patricia Mathias (additional)
Yibing Wang (additional)
Glen Nemerow (additional)

References 36 Referenced 37

10.1038/315190a0
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A B Rickinson, E Kieff Fields Virology, eds B N Fields, D M Knipe, P M Howley (Lippincott–Raven, Philadelphia), pp. 2397–2446 (1996). / Fields Virology by Rickinson A B (1996)
I Penn Transplant Proc 11, 1047–1051 (1979). / Transplant Proc by Penn I (1979)
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E Kieff Fields Virology, eds B N Fields, D M Knipe, P M Howley (Lippincott–Raven, Philadelphia), pp. 2343–2396 (1996). / Fields Virology by Kieff E (1996)
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10.1128/mcb.6.11.3838-3846.1986
10.1128/jvi.63.6.2644-2649.1989
J E Coligan, A M Kruisbeek, D H Margulies, E M Shevach, W Strober Current Protocols in Immunology (Wiley, New York), pp. 7.5.1–7.5.3 (1992). / Current Protocols in Immunology by Coligan J E (1992)
J E Coligan, A M Kruisbeek, D H Margulies, E M Shevach, W Strober Current Protocols in Immunology (Wiley, New York), pp. 7.22.1–7.22.2 (1992). / Current Protocols in Immunology by Coligan J E (1992)
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10.1111/j.1749-6632.1992.tb21063.x
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G Klein Advance in Viral Oncology, ed G Klein (Raven, New York), pp. 207–211 (1987). / Advance in Viral Oncology by Klein G (1987)
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10.1084/jem.159.5.1554
K Bhatia, A Raj, M I Gutiérrez, J G Judde, G Spangler, H Venkatesh, I T Magrath Oncogene 13, 177–181 (1996). / Oncogene by Bhatia K (1996)
10.1073/pnas.91.10.4407
10.1073/pnas.91.13.6196

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:07 p.m.)
Indexed	1 year, 1 month ago (July 11, 2024, 8 a.m.)
Issued	28 years, 1 month ago (July 22, 1997)
Published	28 years, 1 month ago (July 22, 1997)
Published Online	28 years, 1 month ago (July 22, 1997)
Published Print	28 years, 1 month ago (July 22, 1997)

Funders 0

None

BibTeX

@article{Huang_1997, title={Growth arrest of Epstein–Barr virus immortalized B lymphocytes by adenovirus-delivered ribozymes}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.15.8156}, DOI={10.1073/pnas.94.15.8156}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Huang, Shuang and Stupack, Dwayne and Mathias, Patricia and Wang, Yibing and Nemerow, Glen}, year={1997}, month=jul, pages={8156–8161} }

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