DOI: 10.1073/pnas.94.15.7897. The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3′-end formation

The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3′-end formation

10.1073/pnas.94.15.7897

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Polyadenylation of premessenger RNAs occurs posttranscriptionally in the nucleus of eukaryotic cells by cleavage of the precursor and polymerization of adenosine residues. In the yeast Saccharomyces cerevisiae , the mature poly(A) tail ranges from 60 to 70 nucleotides. 3′-end processing can be reproduced in vitro with purified factors. The cleavage reaction requires cleavage factors I and II (CF I and CF II), whereas polyadenylation involves CF I, polyadenylation factor I (PFI), and poly(A) polymerase (Pap1p). CF I has recently been separated into two factors, CF IA and CF IB. We have independently purified CF IA and found that five polypeptides cofractionate with the activity. They include Rna14p, Rna15p, Pcf11p, a new protein called Clp1p, and remarkably, the major poly(A)-binding protein Pab1p. Extracts from strains where the PAB1 gene is mutated or deleted are active for cleavage but generate transcripts bearing abnormally long poly(A) tracts. Complementation with recombinant Pab1p not only restores the length of the poly(A) tails to normal, but also triggers a poly(A) shortening activity. In addition, a monoclonal Pab1p antibody prevents the formation of poly(A) tails in extracts or in a reconstituted system. Our data support the notion that Pab1p is involved in the length control of the poly(A) tails of yeast mRNAs and define a new essential function for Pab1p in the formation of mature mRNAs.

Bibliography

Minvielle-Sebastia, L., Preker, P. J., Wiederkehr, T., Strahm, Y., & Keller, W. (1997). The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3â²-endâformation. Proceedings of the National Academy of Sciences, 94(15), 7897â7902.

Authors 5

Lionel Minvielle-Sebastia (first)
Pascal J. Preker (additional)
Thomas Wiederkehr (additional)
Yvan Strahm (additional)
Walter Keller (additional)

References 30 Referenced 122

10.1016/S0968-0004(96)10030-X
10.1146/annurev.bi.61.070192.002223
10.1016/0959-437X(95)80012-3
10.1016/S0968-0004(96)10057-8
10.1126/science.2848317
J S Butler, P P Sadhale, T Platt Mol Cell Biol 10, 2599–2605 (1990). / Mol Cell Biol by Butler J S (1990)
10.1128/mcb.12.8.3470-3481.1992
10.1016/S0021-9258(18)31508-4
10.1038/354496a0
10.1126/science.7992054
10.1074/jbc.271.43.27167
10.1128/MCB.17.3.1102
10.1016/0092-8674(86)90557-X
10.1016/0092-8674(89)90938-0
10.1016/0092-8674(92)90246-9
10.1101/gad.9.23.2997
10.1002/j.1460-2075.1996.tb01108.x
10.1101/gad.9.19.2421
10.1074/jbc.271.1.432
10.1128/MCB.16.10.5744
10.1128/mcb.7.9.3268-3276.1987
J T Anderson, M R Paddy, M S Swanson Mol Cell Biol 13, 6102–6113 (1993). / Mol Cell Biol by Anderson J T (1993)
10.1016/0092-8674(95)90391-7
Preker P. J. Ohnacker M. Minvielle-Sebastia L. & Keller W. (1997) EMBO J. in press.
10.1016/S0955-0674(97)80004-X
10.1074/jbc.270.6.2800
10.1016/0092-8674(91)90119-J
10.1016/S0021-9258(18)53864-3
10.1101/gad.7.8.1632
10.1016/0968-0004(94)90073-6

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:14 p.m.)
Indexed	2 weeks, 4 days ago (Aug. 5, 2025, 8:36 a.m.)
Issued	28 years, 1 month ago (July 22, 1997)
Published	28 years, 1 month ago (July 22, 1997)
Published Online	28 years, 1 month ago (July 22, 1997)
Published Print	28 years, 1 month ago (July 22, 1997)

Funders 0

None

BibTeX

@article{Minvielle_Sebastia_1997, title={The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3′-end formation}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.15.7897}, DOI={10.1073/pnas.94.15.7897}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Minvielle-Sebastia, Lionel and Preker, Pascal J. and Wiederkehr, Thomas and Strahm, Yvan and Keller, Walter}, year={1997}, month=jul, pages={7897–7902} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T12:36:11Z",
    "timestamp": 1754397371432
  },
  "reference-count": 30,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "15",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1997,
        7,
        22
      ]
    ]
  },
  "abstract": "<jats:p>\n            Polyadenylation of premessenger RNAs occurs posttranscriptionally in the nucleus of eukaryotic cells by cleavage of the precursor and polymerization of adenosine residues. In the yeast\n            <jats:italic>Saccharomyces cerevisiae</jats:italic>\n            , the mature poly(A) tail ranges from 60 to 70 nucleotides. 3\u2032-end processing can be reproduced\n            <jats:italic>in vitro</jats:italic>\n            with purified factors. The cleavage reaction requires cleavage factors I and II (CF I and CF II), whereas polyadenylation involves CF I, polyadenylation factor I (PFI), and poly(A) polymerase (Pap1p). CF I has recently been separated into two factors, CF IA and CF IB. We have independently purified CF IA and found that five polypeptides cofractionate with the activity. They include Rna14p, Rna15p, Pcf11p, a new protein called Clp1p, and remarkably, the major poly(A)-binding protein Pab1p. Extracts from strains where the\n            <jats:italic>PAB1</jats:italic>\n            gene is mutated or deleted are active for cleavage but generate transcripts bearing abnormally long poly(A) tracts. Complementation with recombinant Pab1p not only restores the length of the poly(A) tails to normal, but also triggers a poly(A) shortening activity. In addition, a monoclonal Pab1p antibody prevents the formation of poly(A) tails in extracts or in a reconstituted system. Our data support the notion that Pab1p is involved in the length control of the poly(A) tails of yeast mRNAs and define a new essential function for Pab1p in the formation of mature mRNAs.\n          </jats:p>",
  "DOI": "10.1073/pnas.94.15.7897",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:31:44Z",
    "timestamp": 1027693904000
  },
  "page": "7897-7902",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 122,
  "title": "The major yeast poly(A)-binding protein is associated with cleavage factor IA and functions in premessenger RNA 3\u2032-end\u2009formation",
  "prefix": "10.1073",
  "volume": "94",
  "author": [
    {
      "given": "Lionel",
      "family": "Minvielle-Sebastia",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland"
        }
      ]
    },
    {
      "given": "Pascal J.",
      "family": "Preker",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland"
        }
      ]
    },
    {
      "given": "Thomas",
      "family": "Wiederkehr",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland"
        }
      ]
    },
    {
      "given": "Yvan",
      "family": "Strahm",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland"
        }
      ]
    },
    {
      "given": "Walter",
      "family": "Keller",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1997,
        7,
        22
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0968-0004(96)10030-X"
    },
    {
      "key": "e_1_3_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.61.070192.002223"
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0959-437X(95)80012-3"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0968-0004(96)10057-8"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.2848317"
    },
    {
      "key": "e_1_3_3_6_2",
      "first-page": "2599",
      "volume": "10",
      "author": "Butler J S",
      "year": "1990",
      "unstructured": "J S Butler, P P Sadhale, T Platt Mol Cell Biol 10, 2599\u20132605 (1990).",
      "journal-title": "Mol Cell Biol"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.12.8.3470-3481.1992"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)31508-4"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/354496a0"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7992054"
    },
    {
      "key": "e_1_3_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.43.27167"
    },
    {
      "key": "e_1_3_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.17.3.1102"
    },
    {
      "key": "e_1_3_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90557-X"
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90938-0"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90246-9"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.9.23.2997"
    },
    {
      "key": "e_1_3_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb01108.x"
    },
    {
      "key": "e_1_3_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.9.19.2421"
    },
    {
      "key": "e_1_3_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.1.432"
    },
    {
      "key": "e_1_3_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.16.10.5744"
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.7.9.3268-3276.1987"
    },
    {
      "key": "e_1_3_3_22_2",
      "first-page": "6102",
      "volume": "13",
      "author": "Anderson J T",
      "year": "1993",
      "unstructured": "J T Anderson, M R Paddy, M S Swanson Mol Cell Biol 13, 6102\u20136113 (1993).",
      "journal-title": "Mol Cell Biol"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90391-7"
    },
    {
      "key": "e_1_3_3_24_2",
      "unstructured": "Preker P.\u2009J. Ohnacker M. Minvielle-Sebastia L. & Keller W. (1997) EMBO J. in press."
    },
    {
      "key": "e_1_3_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(97)80004-X"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.6.2800"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90119-J"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)53864-3"
    },
    {
      "key": "e_1_3_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.7.8.1632"
    },
    {
      "key": "e_1_3_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0968-0004(94)90073-6"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.94.15.7897",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T19:14:51Z",
    "timestamp": 1649877291000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.94.15.7897"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1997,
        7,
        22
      ]
    ]
  },
  "references-count": 30,
  "journal-issue": {
    "issue": "15",
    "published-print": {
      "date-parts": [
        [
          1997,
          7,
          22
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.94.15.7897"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.94.15.7897",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1997,
        7,
        22
      ]
    ]
  },
  "assertion": [
    {
      "value": "1997-02-14",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1997-05-19",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1997-07-22",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}