DOI: 10.1073/pnas.94.13.7041. Involvement of hippocampal cAMP/cAMP-dependent protein kinase signaling pathways in a late memory consolidation phase of aversively motivated learning in rats

Involvement of hippocampal cAMP/cAMP-dependent protein kinase signaling pathways in a late memory consolidation phase of aversively motivated learning in rats

10.1073/pnas.94.13.7041

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

cAMP/cAMP-dependent protein kinase (PKA) signaling pathway has been recently proposed to participate in both the late phase of long term potentiation in the hippocampus and in the late, protein synthesis-dependent phase of memory formation. Here we report that a late memory consolidation phase of an inhibitory avoidance learning is regulated by an hippocampal cAMP signaling pathway that is activated, at least in part, by D 1 /D 5 receptors. Bilateral infusion of SKF 38393 (7.5 μg/side), a D 1 /D 5 receptor agonist, into the CA 1 region of the dorsal hippocampus, enhanced retention of a step-down inhibitory avoidance when given 3 or 6 h, but not immediately (0 h) or 9 h, after training. In contrast, full retrograde amnesia was obtained when SCH 23390 (0.5 μg/side), a D 1 /D 5 receptor antagonist, was infused into the hippocampus 3 or 6 h after training. Intrahippocampal infusion of 8Br-cAMP (1.25 μg/side), or forskolin (0.5 μg/side), an activator of adenylyl cyclase, enhanced memory when given 3 or 6 h after training. KT5720 (0.5 μg/side), a specific inhibitor of PKA, hindered memory consolidation when given immediately or 3 or 6 h posttraining. Rats submitted to the avoidance task showed learning-specific increases in hippocampal 3 H-SCH 23390 binding and in the endogenous levels of cAMP 3 and 6 h after training. In addition, PKA activity and P-CREB (phosphorylated form of cAMP responsive element binding protein) immunoreactivity increased in the hippocampus immediately and 3 and 6 h after training. Together, these findings suggest that the late phase of memory consolidation of an inhibitory avoidance is modulated cAMP/PKA signaling pathways in the hippocampus.

Bibliography

Bernabeu, R., Bevilaqua, L., Ardenghi, P., Bromberg, E., Schmitz, P., Bianchin, M., Izquierdo, I., & Medina, J. H. (1997). Involvement of hippocampal cAMP/cAMP-dependent protein kinase signaling pathways in a late memory consolidation phase of aversively motivated learning inârats. Proceedings of the National Academy of Sciences, 94(13), 7041â7046.

Authors 8

Ramon Bernabeu (first)
Lia Bevilaqua (additional)
Patricia Ardenghi (additional)
Elke Bromberg (additional)
Paulo Schmitz (additional)
Marino Bianchin (additional)
Ivan Izquierdo (additional)
Jorge H. Medina (additional)

References 50 Referenced 414

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:44 p.m.)
Indexed	1 month ago (July 30, 2025, 11:20 a.m.)
Issued	28 years, 2 months ago (June 24, 1997)
Published	28 years, 2 months ago (June 24, 1997)
Published Online	28 years, 2 months ago (June 24, 1997)
Published Print	28 years, 2 months ago (June 24, 1997)

Funders 0

None

BibTeX

@article{Bernabeu_1997, title={Involvement of hippocampal cAMP/cAMP-dependent protein kinase signaling pathways in a late memory consolidation phase of aversively motivated learning in rats}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.13.7041}, DOI={10.1073/pnas.94.13.7041}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Bernabeu, Ramon and Bevilaqua, Lia and Ardenghi, Patricia and Bromberg, Elke and Schmitz, Paulo and Bianchin, Marino and Izquierdo, Ivan and Medina, Jorge H.}, year={1997}, month=jun, pages={7041–7046} }

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