DOI: 10.1073/pnas.94.12.6042. The 2.1-Å crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box

The 2.1-Å crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box

10.1073/pnas.94.12.6042

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Archaea possess a basal transcriptional apparatus that resembles that of eukaryotes. Here we report the 2.1-Å crystal structure of the archaeal transcription factor complex formed by the TATA-box-binding protein (TBP), the transcription factor IIB homolog, and a DNA target, all from the hyperthermophile Pyrococcus woesei . The overall fold of these two basal transcription factors is essentially the same as that of their eukaryotic counterparts. However, in comparison with the eukaryotic complexes, the archaeal TBP–DNA interface is more symmetrical, and in this structure the orientation of the preinitiation complex assembly on the promoter is inverted with respect to that seen in all crystal structures of comparable eukaryotic systems. This study of the structural details of an archaeal transcription factor complex presents the opportunity to examine the evolution of the basal eukaryotic transcriptional apparatus from a stereochemical viewpoint and to extend our understanding of the physical biochemistry of transcriptional initiation.

Bibliography

Kosa, P. F., Ghosh, G., DeDecker, B. S., & Sigler, P. B. (1997). The 2.1-â« crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box. Proceedings of the National Academy of Sciences, 94(12), 6042â6047.

Authors 4

Peter F. Kosa (first)
Gourishankar Ghosh (additional)
Brian S. DeDecker (additional)
Paul B. Sigler (additional)

References 31 Referenced 130

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 2 months ago (June 6, 2022, 5:22 p.m.)
Indexed	4 weeks, 1 day ago (July 30, 2025, 9:54 a.m.)
Issued	28 years, 2 months ago (June 10, 1997)
Published	28 years, 2 months ago (June 10, 1997)
Published Online	28 years, 2 months ago (June 10, 1997)
Published Print	28 years, 2 months ago (June 10, 1997)

Funders 0

None

BibTeX

@article{Kosa_1997, title={The 2.1-Å crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.12.6042}, DOI={10.1073/pnas.94.12.6042}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kosa, Peter F. and Ghosh, Gourishankar and DeDecker, Brian S. and Sigler, Paul B.}, year={1997}, month=jun, pages={6042–6047} }

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