DOI: 10.1073/pnas.94.11.5709. The origin and efficient derivation of embryonic stem cells in the mouse

The origin and efficient derivation of embryonic stem cells in the mouse

10.1073/pnas.94.11.5709

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

By explanting tissues isolated microsurgically from implanting strain 129 mouse blastocysts individually on STO feeder cells we have established that embryonic stem (ES) cells originate from the epiblast (primitive ectoderm). Isolated early epiblasts yielded ES cell lines at a substantially higher frequency than intact blastocysts regardless of whether they were explanted whole or as strictly single-cell suspensions. When explanted from delayed-implanting 129 blastocysts, epiblasts gave lines consistently in 100% of cases. If primary embryonic fibroblasts rather than STO cells were used as feeders, germline-competent ES cell lines were obtained readily from epiblasts of delayed-implanting blastocysts of several hitherto refractory strains, particularly when recombinant leukemia inhibitory factor was included in the medium during the initial period of culture. Because lines were obtained from the nonpermissive CBA/Ca strain at a rate of up to 56%, this approach to the derivation of germline-competent ES cell lines may not only prove generic for the mouse but also worth pursuing in other species of mammal.

Bibliography

Brook, F. A., & Gardner, R. L. (1997). The origin and efficient derivation of embryonic stem cells in theâmouse. Proceedings of the National Academy of Sciences, 94(11), 5709â5712.

Authors 2

F. A. Brook (first)
R. L. Gardner (additional)

References 25 Referenced 419

Gardner R. L. & Brook F. A. (1997) Int. J. Dev. Biol. in press.
10.1111/j.1440-169X.1989.00275.x
10.1038/292154a0
10.1073/pnas.78.12.7634
10.1007/BF00376370
R L Gardner J Embryol Exp Morph 88, 303–326 (1985). / J Embryol Exp Morph by Gardner R L (1985)
M L Hooper Embryonal Stem Cells: Introducing Planned Changes into the Animal Germline (Harwood, Switzerland, 1992). / Embryonal Stem Cells: Introducing Planned Changes into the Animal Germline by Hooper M L (1992)
10.1002/ijc.2910130403
10.1242/dev.111.1.75
E J Robertson Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, ed E J Robertson (IRL, Oxford), pp. 71–112 (1987). / Teratocarcinomas and Embryonic Stem Cells: A Practical Approach by Robertson E J (1987)
10.1038/ng1096-223
10.1101/gad.5.9.1513
10.1093/oxfordjournals.humrep.a138039
R L Gardner, J Rossant J Embryol Exp Morph 52, 141–152 (1979). / J Embryol Exp Morph by Gardner R L (1979)
10.1017/S001667230003531X
10.1126/science.2563174
B Hogan, F Constantini, E Lacy Manipulating the Mouse Embryo: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 221–225 (1986). / Manipulating the Mouse Embryo: A Laboratory Manual by Hogan B (1986)
A Bradley Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, ed E J Robertson (IRL, Oxford), pp. 113–151 (1987). / Teratocarcinomas and Embryonic Stem Cells: A Practical Approach by Bradley A (1987)
10.1016/B978-0-12-201850-3.50011-5
10.1002/mrd.1120120308
10.1111/j.1440-169X.1981.00157.x
10.1101/gad.8.20.2466
10.1016/0076-6879(93)25052-4
M J Evans, M H Kaufman Cancer Surv 2, 185–207 (1983). / Cancer Surv by Evans M J (1983)
10.1002/jez.1402650108

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:32 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:28 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 12, 2025, 6:09 p.m.)
Issued	28 years, 3 months ago (May 27, 1997)
Published	28 years, 3 months ago (May 27, 1997)
Published Online	28 years, 3 months ago (May 27, 1997)
Published Print	28 years, 3 months ago (May 27, 1997)

Funders 0

None

BibTeX

@article{Brook_1997, title={The origin and efficient derivation of embryonic stem cells in the mouse}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.11.5709}, DOI={10.1073/pnas.94.11.5709}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Brook, F. A. and Gardner, R. L.}, year={1997}, month=may, pages={5709–5712} }

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