DOI: 10.1073/pnas.94.11.5605. BRCA1 is a component of the RNA polymerase II holoenzyme

BRCA1 is a component of the RNA polymerase II holoenzyme

10.1073/pnas.94.11.5605

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The familial breast–ovarian tumor suppressor gene product BRCA1 was found to be a component of the RNA polymerase II holoenzyme by several criteria. BRCA1 was found to copurify with the holoenzyme over multiple chromatographic steps. Other tested transcription activators that could potentially contact the holoenzyme were not stably associated with the holoenzyme as determined by copurification. Antibody specific for the holoenzyme component hSRB7 specifically purifies BRCA1. Immunopurification of BRCA1 complexes also specifically purifies transcriptionally active RNA polymerase II and transcription factors TFIIF, TFIIE, and TFIIH. Moreover, a BRCA1 domain, which is deleted in about 90% of clinically relevant mutations, participates in binding to the holoenzyme complex in cells. These data are consistent with recent data identifying transcription activation domains in the BRCA1 protein and link the BRCA1 tumor suppressor protein with the transcription process as a holoenzyme-bound protein.

Bibliography

Scully, R., Anderson, S. F., Chao, D. M., Wei, W., Ye, L., Young, R. A., Livingston, D. M., & Parvin, J. D. (1997). BRCA1 is a component of the RNA polymeraseâIIâholoenzyme. Proceedings of the National Academy of Sciences, 94(11), 5605â5610.

Authors 8

Ralph Scully (first)
Stephen F. Anderson (additional)
David M. Chao (additional)
Wanjiang Wei (additional)
Liyan Ye (additional)
Richard A. Young (additional)
David M. Livingston (additional)
Jeffrey D. Parvin (additional)

References 32 Referenced 346

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:34 p.m.)
Indexed	1 month ago (July 22, 2025, 6:32 a.m.)
Issued	28 years, 2 months ago (May 27, 1997)
Published	28 years, 2 months ago (May 27, 1997)
Published Online	28 years, 2 months ago (May 27, 1997)
Published Print	28 years, 2 months ago (May 27, 1997)

Funders 0

None

BibTeX

@article{Scully_1997, title={BRCA1 is a component of the RNA polymerase II holoenzyme}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.11.5605}, DOI={10.1073/pnas.94.11.5605}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Scully, Ralph and Anderson, Stephen F. and Chao, David M. and Wei, Wanjiang and Ye, Liyan and Young, Richard A. and Livingston, David M. and Parvin, Jeffrey D.}, year={1997}, month=may, pages={5605–5610} }

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