DOI: 10.1073/pnas.94.10.5012. The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of the F <sub>1</sub> -ATPase

The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of the F 1 -ATPase

10.1073/pnas.94.10.5012

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Bacteriophage T7 DNA helicase is a ring-shaped hexamer that catalyzes duplex DNA unwinding using dTTP hydrolysis as an energy source. Of the six potential nucleotide binding sites on the hexamer, we have found that three are noncatalytic sites and three are catalytic sites. The noncatalytic sites bind nucleotides with a high affinity, but dTTPs bound to these sites do not dissociate or hydrolyze through many dTTPase turnovers at the catalytic sites. The catalytic sites show strong cooperativity which leads to sequential binding and hydrolysis of dTTP. The elucidated dTTPase mechanism of the catalytic sites of T7 helicase is remarkably similar to the binding change mechanism of the ATP synthase. Based on the similarity, a general mechanism for hexameric helicases is proposed. In this mechanism, an F 1 -ATPase-like rotational movement around the single-stranded DNA, which is bound through the central hole of the hexamer, is proposed to lead to unidirectional translocation along single-stranded DNA and duplex DNA unwinding.

Bibliography

Hingorani, M. M., Washington, M. T., Moore, K. C., & Patel, S. S. (1997). The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of theâF 1 -ATPase. Proceedings of the National Academy of Sciences, 94(10), 5012â5017.

Authors 4

Manju M. Hingorani (first)
M. Todd Washington (additional)
Kristen C. Moore (additional)
Smita S. Patel (additional)

References 31 Referenced 77

10.1111/j.1365-2958.1992.tb00831.x
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:39 p.m.)
Indexed	1 year, 2 months ago (July 1, 2024, 12:33 p.m.)
Issued	28 years, 3 months ago (May 13, 1997)
Published	28 years, 3 months ago (May 13, 1997)
Published Online	28 years, 3 months ago (May 13, 1997)
Published Print	28 years, 3 months ago (May 13, 1997)

Funders 0

None

BibTeX

@article{Hingorani_1997, title={The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of the F 1 -ATPase}, volume={94}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.94.10.5012}, DOI={10.1073/pnas.94.10.5012}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hingorani, Manju M. and Washington, M. Todd and Moore, Kristen C. and Patel, Smita S.}, year={1997}, month=may, pages={5012–5017} }

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