DOI: 10.1073/pnas.93.8.3169. Thiocalsin: a thioredoxin-linked, substrate-specific protease dependent on calcium.

Thiocalsin: a thioredoxin-linked, substrate-specific protease dependent on calcium.

10.1073/pnas.93.8.3169

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We describe a protease, named "thiocalsin," that is activated by calcium but only after reductive activation by thioredoxin, a small protein with a redox-active disulfide group that functions widely in regulation. Thiocalsin appeared to be a 14-kDa serine protease that functions independently of calmodulin. The enzyme, purified from germinating wheat grain, specifically cleaved the major indigenous storage proteins, gliadins and glutenins, after they too had been reduced, preferentially by thioredoxin. The disulfide groups of the enzyme, as well as its protein substrates, were reduced by thioredoxin via NADPH and the associated enzyme, NADP-thioredoxin reductase. The results broaden the roles of thioredoxin and calcium and suggest a joint function in activating thiocalsin, thereby providing amino acids for germination and seedling development.

Bibliography

Besse, I., Wong, J. H., Kobrehel, K., & Buchanan, B. B. (1996). Thiocalsin: a thioredoxin-linked, substrate-specific protease dependent on calcium. Proceedings of the National Academy of Sciences, 93(8), 3169â3175.

Authors 4

I Besse (first)
J H Wong (additional)
K Kobrehel (additional)
B B Buchanan (additional)

References 0 Referenced 77

None

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:19 p.m.)
Indexed	3 weeks, 4 days ago (Aug. 6, 2025, 8:53 a.m.)
Issued	29 years, 4 months ago (April 16, 1996)
Published	29 years, 4 months ago (April 16, 1996)
Published Online	29 years, 4 months ago (April 16, 1996)
Published Print	29 years, 4 months ago (April 16, 1996)

Funders 0

None

BibTeX

@article{Besse_1996, title={Thiocalsin: a thioredoxin-linked, substrate-specific protease dependent on calcium.}, volume={93}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.93.8.3169}, DOI={10.1073/pnas.93.8.3169}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Besse, I and Wong, J H and Kobrehel, K and Buchanan, B B}, year={1996}, month=apr, pages={3169–3175} }

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