DOI: 10.1073/pnas.93.3.1210. A cell cycle-regulated bacterial DNA methyltransferase is essential for viability.

A cell cycle-regulated bacterial DNA methyltransferase is essential for viability.

10.1073/pnas.93.3.1210

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The CcrM adenine DNA methyltransferase, which specifically modifies GANTC sequences, is necessary for viability in Caulobacter crescentus. To our knowledge, this is the first example of an essential prokaryotic DNA methyltransferase that is not part of a DNA restriction/modification system. Homologs of CcrM are widespread in the alpha subdivision of the Proteobacteria, suggesting that methylation at GANTC sites may have important functions in other members of this diverse group as well. Temporal control of DNA methylation state has an important role in Caulobacter development, and we show that this organism utilizes an unusual mechanism for control of remethylation of newly replicated DNA. CcrM is synthesized de novo late in the cell cycle, coincident with full methylation of the chromosome, and is then subjected to proteolysis prior to cell division.

Bibliography

Stephens, C., Reisenauer, A., Wright, R., & Shapiro, L. (1996). A cell cycle-regulated bacterial DNA methyltransferase is essential for viability. Proceedings of the National Academy of Sciences, 93(3), 1210â1214.

Authors 4

C Stephens (first)
A Reisenauer (additional)
R Wright (additional)
L Shapiro (additional)

References 0 Referenced 187

None

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:14 p.m.)
Indexed	1 week, 5 days ago (Aug. 19, 2025, 6:35 a.m.)
Issued	29 years, 6 months ago (Feb. 6, 1996)
Published	29 years, 6 months ago (Feb. 6, 1996)
Published Online	29 years, 6 months ago (Feb. 6, 1996)
Published Print	29 years, 6 months ago (Feb. 6, 1996)

Funders 0

None

BibTeX

@article{Stephens_1996, title={A cell cycle-regulated bacterial DNA methyltransferase is essential for viability.}, volume={93}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.93.3.1210}, DOI={10.1073/pnas.93.3.1210}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Stephens, C and Reisenauer, A and Wright, R and Shapiro, L}, year={1996}, month=feb, pages={1210–1214} }

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