DOI: 10.1073/pnas.93.26.15341. CD8<sup>+</sup>T-cell-derived soluble factor(s), but not β-chemokines RANTES, MIP-1α, and MIP-1β, suppress HIV-1 replication in monocyte/macrophages

CD8+T-cell-derived soluble factor(s), but not β-chemokines RANTES, MIP-1α, and MIP-1β, suppress HIV-1 replication in monocyte/macrophages

10.1073/pnas.93.26.15341

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

It has been demonstrated that CD8+T cells produce a soluble factor(s) that suppresses human immunodeficiency virus (HIV) replication in CD4+T cells. The role of soluble factors in the suppression of HIV replication in monocyte/macrophages (M/M) has not been fully delineated. To investigate whether a CD8+T-cell-derived soluble factor(s) can also suppress HIV infection in the M/M system, primary macrophages were infected with the macrophage tropic HIV-1 strain Ba-L. CD8+T-cell-depleted peripheral blood mononuclear cells were also infected with HIV-1 IIIB or Ba-L. HIV expression from the chronically infected macrophage cell line U1 was also determined in the presence of CD8+T-cell supernatants or β-chemokines. We demonstrate that: (i) CD8+T-cell supernatants did, but β-chemokines did not, suppress HIV replication in the M/M system; (ii) antibodies to regulated on activation normal T-cell expressed and Secreted (RANTES), macrophage inflammatory protein 1α (MIP-1α) and MIP-1β did not, whereas antibodies to interleukin 10, interleukin 13, interferon α, or interferon γ modestly reduced anti-HIV activity of the CD8+T-cell supernatants; and (iii) the CD8+T-cell supernatants did, but β-chemokines did not, suppress HIV-1 IIIB replication in peripheral blood mononuclear cells as well as HIV expression in U1 cells. These results suggest that HIV-suppressor activity of CD8+T cells is a multifactorial phenomenon, and that RANTES, MIP-1α, and MIP-1β do not account for the entire scope of CD8+T-cell-derived HIV-suppressor factors.

Bibliography

Moriuchi, H., Moriuchi, M., Combadiere, C., Murphy, P. M., & Fauci, A. S. (1996). CD8+T-cell-derived soluble factor(s), but not Î²-chemokines RANTES, MIP-1Î±, and MIP-1Î², suppress HIV-1 replication inâmonocyte/macrophages. Proceedings of the National Academy of Sciences, 93(26), 15341â15345.

Authors 5

Hiroyuki Moriuchi (first)
Masako Moriuchi (additional)
Christophe Combadiere (additional)
Philip M. Murphy (additional)
Anthony S. Fauci (additional)

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Dates

Type	When
Created	23 years ago (July 26, 2002, 10:34 a.m.)
Deposited	2 years, 4 months ago (April 22, 2023, 8:13 a.m.)
Indexed	1 month, 3 weeks ago (June 30, 2025, 7:26 a.m.)
Issued	28 years, 7 months ago (Dec. 24, 1996)
Published	28 years, 7 months ago (Dec. 24, 1996)
Published Online	28 years, 7 months ago (Dec. 24, 1996)
Published Print	28 years, 7 months ago (Dec. 24, 1996)

Funders 0

None

BibTeX

@article{Moriuchi_1996, title={CD8+T-cell-derived soluble factor(s), but not β-chemokines RANTES, MIP-1α, and MIP-1β, suppress HIV-1 replication in monocyte/macrophages}, volume={93}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.93.26.15341}, DOI={10.1073/pnas.93.26.15341}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Moriuchi, Hiroyuki and Moriuchi, Masako and Combadiere, Christophe and Murphy, Philip M. and Fauci, Anthony S.}, year={1996}, month=dec, pages={15341–15345} }

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