DOI: 10.1073/pnas.93.26.15185. Modulation of the chaperone-like activity of bovine α-crystallin

Modulation of the chaperone-like activity of bovine α-crystallin

10.1073/pnas.93.26.15185

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The effects of pantethine, glutathione, and selected chemical reagents on the anti-aggregation activity of α-crystallin was evaluated. Protein aggregation was monitored by light scattering of solutions of denatured β L -crystallin or alcohol dehydrogenase (ADH). The ratios of β L -crystallin/α-crystallin and ADH/α-crystallin were adjusted so that partial inhibition of protein aggregation at 60°C or 37°C, respectively, was observed and modulation of the chaperone action of α-crystallin could be evaluated easily with selected endogenous metabolites. Enhancement of the anti-aggregation activity in the β L -crystallin assay was strongest with pantethine, which appeared to interact with α-crystallin. Enhancement of the anti-aggregation activity in the ADH assay was strongest with glutathione which appeared to interact with ADH. The results indicated that the products of common metabolic pathways can modulate the chaperone-like effects of α-crystallin on protein aggregation.

Bibliography

Clark, J. I., & Huang, Q. (1996). Modulation of the chaperone-like activity of bovineâÎ±-crystallin. Proceedings of the National Academy of Sciences, 93(26), 15185â15189.

Authors 2

John I. Clark (first)
Qing-ling Huang (additional)

References 39 Referenced 50

10.1073/pnas.89.21.10449
10.1016/S0021-9258(17)36828-X
10.1016/0304-4165(95)00125-5
K Wang, A Spector Invest Ophthalmol Visual Sci 36, 311–321 (1995). / Invest Ophthalmol Visual Sci by Wang K (1995)
10.1016/S0021-9258(17)36872-2
10.1016/S0021-9258(18)54039-4
10.1016/S0021-9258(18)53882-5
W W de-Jong, J A Leunissen, C E Voorter Mol Biol Evol 10, 103–126 (1993). / Mol Biol Evol by de-Jong W W (1993)
10.1016/S0006-291X(89)80215-3
10.1111/j.1432-1033.1991.tb16432.x
10.1016/0092-8674(89)90173-6
10.1016/0167-4838(91)90146-Q
10.1128/mcb.11.2.803-812.1991
10.1006/jmbi.1993.1476
10.1016/S0021-9258(18)50096-X
10.1016/0014-5793(89)80022-5
10.1128/mcb.9.3.1083-1091.1989
10.1016/0006-291X(92)91809-5
10.1016/0014-4835(92)90058-Z
10.1007/BF00221054
10.1016/0140-6736(90)92075-S
10.1128/mcb.11.8.3905-3914.1991
10.1016/S0014-4835(05)80208-8
10.1038/375739b0
10.1006/bbrc.1995.1985
10.1006/bbrc.1995.1954
10.1006/bbrc.1995.1391
10.1016/S0014-4835(05)80136-8
10.1016/0014-5793(88)80295-3
J A Castoro, F A Bettelheim Lens Eye Toxic Res 6, 781–793 (1989). / Lens Eye Toxic Res by Castoro J A (1989)
10.1016/0167-4838(93)90107-3
10.1021/bi00030a001
10.1016/0014-5793(95)00440-K
10.1016/0014-5793(95)00775-5
T Hiraoka, J I Clark Invest Ophthalmol Visual Sci 36, 2550–2555 (1995). / Invest Ophthalmol Visual Sci by Hiraoka T (1995)
10.1006/exer.1996.0002
10.1096/fasebj.10.1.8566552
10.1016/0167-4838(95)00146-L
10.1111/j.1432-1033.1994.tb20021.x

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:36 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 1:51 p.m.)
Issued	28 years, 8 months ago (Dec. 24, 1996)
Published	28 years, 8 months ago (Dec. 24, 1996)
Published Online	28 years, 8 months ago (Dec. 24, 1996)
Published Print	28 years, 8 months ago (Dec. 24, 1996)

Funders 0

None

BibTeX

@article{Clark_1996, title={Modulation of the chaperone-like activity of bovine α-crystallin}, volume={93}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.93.26.15185}, DOI={10.1073/pnas.93.26.15185}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Clark, John I. and Huang, Qing-ling}, year={1996}, month=dec, pages={15185–15189} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T17:51:16Z",
    "timestamp": 1751478676939
  },
  "reference-count": 39,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "26",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1996,
        12,
        24
      ]
    ]
  },
  "abstract": "<jats:p>\n            The effects of pantethine, glutathione, and selected chemical\n reagents on the anti-aggregation activity of \u03b1-crystallin was\n evaluated. Protein aggregation was monitored by light scattering of\n solutions of denatured \u03b2\n            <jats:sub>L</jats:sub>\n            -crystallin or alcohol\n dehydrogenase (ADH). The ratios of\n \u03b2\n            <jats:sub>L</jats:sub>\n            -crystallin/\u03b1-crystallin and\n ADH/\u03b1-crystallin were adjusted so that partial inhibition\n of protein aggregation at 60\u00b0C or 37\u00b0C, respectively, was observed\n and modulation of the chaperone action of \u03b1-crystallin could be\n evaluated easily with selected endogenous metabolites.\n Enhancement of the anti-aggregation activity in the\n \u03b2\n            <jats:sub>L</jats:sub>\n            -crystallin assay was strongest with pantethine, which\n appeared to interact with \u03b1-crystallin. Enhancement of the\n anti-aggregation activity in the ADH assay was strongest with\n glutathione which appeared to interact with ADH. The results indicated\n that the products of common metabolic pathways can modulate the\n chaperone-like effects of \u03b1-crystallin on protein aggregation.\n          </jats:p>",
  "DOI": "10.1073/pnas.93.26.15185",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:40:52Z",
    "timestamp": 1027694452000
  },
  "page": "15185-15189",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 50,
  "title": "Modulation of the chaperone-like activity of bovine\u2009\u03b1-crystallin",
  "prefix": "10.1073",
  "volume": "93",
  "author": [
    {
      "given": "John\u2009I.",
      "family": "Clark",
      "sequence": "first",
      "affiliation": [
        {
          "name": "357420 Biological Structure and Ophthalmology, University of\r Washington, Seattle, WA 98195-7420; and Jules Stein Institute,\r University of California, Los Angeles, School of Medicine, Los\r Angeles, CA 90024-7008"
        }
      ]
    },
    {
      "given": "Qing-ling",
      "family": "Huang",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "357420 Biological Structure and Ophthalmology, University of\r Washington, Seattle, WA 98195-7420; and Jules Stein Institute,\r University of California, Los Angeles, School of Medicine, Los\r Angeles, CA 90024-7008"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1996,
        12,
        24
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_2_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.21.10449"
    },
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)36828-X"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4165(95)00125-5"
    },
    {
      "key": "e_1_3_2_4_2",
      "first-page": "311",
      "volume": "36",
      "author": "Wang K",
      "year": "1995",
      "unstructured": "K Wang, A Spector Invest Ophthalmol Visual Sci 36, 311\u2013321 (1995).",
      "journal-title": "Invest Ophthalmol Visual Sci"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)36872-2"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)54039-4"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)53882-5"
    },
    {
      "key": "e_1_3_2_8_2",
      "first-page": "103",
      "volume": "10",
      "author": "de-Jong W W",
      "year": "1993",
      "unstructured": "W W de-Jong, J A Leunissen, C E Voorter Mol Biol Evol 10, 103\u2013126 (1993).",
      "journal-title": "Mol Biol Evol"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-291X(89)80215-3"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1991.tb16432.x"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90173-6"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4838(91)90146-Q"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.11.2.803-812.1991"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jmbi.1993.1476"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)50096-X"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(89)80022-5"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.9.3.1083-1091.1989"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(92)91809-5"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-4835(92)90058-Z"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00221054"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0140-6736(90)92075-S"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.11.8.3905-3914.1991"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-4835(05)80208-8"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/375739b0"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/bbrc.1995.1985"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/bbrc.1995.1954"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/bbrc.1995.1391"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-4835(05)80136-8"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(88)80295-3"
    },
    {
      "key": "e_1_3_2_30_2",
      "first-page": "781",
      "volume": "6",
      "author": "Castoro J A",
      "year": "1989",
      "unstructured": "J A Castoro, F A Bettelheim Lens Eye Toxic Res 6, 781\u2013793 (1989).",
      "journal-title": "Lens Eye Toxic Res"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4838(93)90107-3"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00030a001"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(95)00440-K"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(95)00775-5"
    },
    {
      "key": "e_1_3_2_35_2",
      "first-page": "2550",
      "volume": "36",
      "author": "Hiraoka T",
      "year": "1995",
      "unstructured": "T Hiraoka, J I Clark Invest Ophthalmol Visual Sci 36, 2550\u20132555 (1995).",
      "journal-title": "Invest Ophthalmol Visual Sci"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/exer.1996.0002"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1096/fasebj.10.1.8566552"
    },
    {
      "key": "e_1_3_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4838(95)00146-L"
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1994.tb20021.x"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.93.26.15185",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T18:36:02Z",
    "timestamp": 1649874962000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.93.26.15185"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1996,
        12,
        24
      ]
    ]
  },
  "references-count": 39,
  "journal-issue": {
    "issue": "26",
    "published-print": {
      "date-parts": [
        [
          1996,
          12,
          24
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.93.26.15185"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.93.26.15185",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1996,
        12,
        24
      ]
    ]
  },
  "assertion": [
    {
      "value": "1996-06-04",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1996-09-30",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1996-12-24",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}