DOI: 10.1073/pnas.93.24.14146. Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca 2+ entry

Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca 2+ entry

10.1073/pnas.93.24.14146

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Capacitative Ca 2+ entry is a component of the inositol-lipid signaling in which depletion of inositol 1,4,5-trisphosphate (Ins P 3 )-sensitive Ca 2+ stores activates Ca 2+ influx by a mechanism that is still unknown. This pathway plays a central role in cellular signaling, which is mediated by many hormones, neurotransmitters, and growth factors. Studies of Drosophila photoreceptors provided the first putative capacitative Ca 2+ entry mutant designated transient receptor potential ( trp ) and a Drosophila gene encoding TRP-like protein ( trpl ). It is not clear how the Ca 2+ store depletion signal is relayed to the plasma membrane and whether both TRP and TRPL participate in this process. We report here that coexpressing Drosophila TRP and TRPL in Xenopus oocytes synergistically enhances the endogenous Ca 2+ -activated Cl − current and produces a divalent inward current. Both of these currents are activated by Ca 2+ store depletion. In the absence of Ca 2+ , Mg 2+ is the main charge carrier of the divalent current. This current is characterized by lanthanum sensitivity and a voltage-dependent blocking effect of Mg 2+ , which is relieved at both hyperpolarizing (inward rectification) and depolarizing (outward rectification) potentials. The store-operated divalent current is neither observed in native oocytes nor in oocytes expressing either TRP or TRPL alone. The production of this current implicates a cooperative action of TRP and TRPL in the depletion-activated current.

Bibliography

Gillo, B., Chorna, I., Cohen, H., Cook, B., Manistersky, I., Chorev, M., Arnon, A., Pollock, J. A., Selinger, Z., & Minke, B. (1996). Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca 2+ âentry. Proceedings of the National Academy of Sciences, 93(24), 14146â14151.

Authors 10

B. Gillo (first)
I. Chorna (additional)
H. Cohen (additional)
B. Cook (additional)
I. Manistersky (additional)
M. Chorev (additional)
A. Arnon (additional)
J. A. Pollock (additional)
Z. Selinger (additional)
B. Minke (additional)

References 52 Referenced 88

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:02 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 2, 2025, 12:33 a.m.)
Issued	28 years, 9 months ago (Nov. 26, 1996)
Published	28 years, 9 months ago (Nov. 26, 1996)
Published Online	28 years, 9 months ago (Nov. 26, 1996)
Published Print	28 years, 9 months ago (Nov. 26, 1996)

Funders 0

None

BibTeX

@article{Gillo_1996, title={Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca 2+ entry}, volume={93}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.93.24.14146}, DOI={10.1073/pnas.93.24.14146}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gillo, B. and Chorna, I. and Cohen, H. and Cook, B. and Manistersky, I. and Chorev, M. and Arnon, A. and Pollock, J. A. and Selinger, Z. and Minke, B.}, year={1996}, month=nov, pages={14146–14151} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T04:33:05Z",
    "timestamp": 1754109185592
  },
  "reference-count": 52,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "24",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1996,
        11,
        26
      ]
    ]
  },
  "abstract": "<jats:p>\n            Capacitative Ca\n            <jats:sup>2+</jats:sup>\n            entry is a component of the\n inositol-lipid signaling in which depletion of inositol\n 1,4,5-trisphosphate (Ins\n            <jats:italic>P</jats:italic>\n            <jats:sub>3</jats:sub>\n            )-sensitive\n Ca\n            <jats:sup>2+</jats:sup>\n            stores activates Ca\n            <jats:sup>2+</jats:sup>\n            influx by a\n mechanism that is still unknown. This pathway plays a central role in\n cellular signaling, which is mediated by many hormones,\n neurotransmitters, and growth factors. Studies of\n            <jats:italic>Drosophila</jats:italic>\n            photoreceptors provided the first putative\n capacitative Ca\n            <jats:sup>2+</jats:sup>\n            entry mutant designated transient\n receptor potential (\n            <jats:italic>trp</jats:italic>\n            ) and a\n            <jats:italic>Drosophila</jats:italic>\n            gene encoding TRP-like protein (\n            <jats:italic>trpl</jats:italic>\n            ). It is not clear\n how the Ca\n            <jats:sup>2+</jats:sup>\n            store depletion signal is relayed to the\n plasma membrane and whether both TRP and TRPL participate in this\n process. We report here that coexpressing\n            <jats:italic>Drosophila</jats:italic>\n            TRP\n and TRPL in\n            <jats:italic>Xenopus</jats:italic>\n            oocytes synergistically enhances the\n endogenous Ca\n            <jats:sup>2+</jats:sup>\n            -activated Cl\n            <jats:sup>\u2212</jats:sup>\n            current and produces a divalent inward current. Both of these currents\n are activated by Ca\n            <jats:sup>2+</jats:sup>\n            store depletion. In the absence of\n Ca\n            <jats:sup>2+</jats:sup>\n            , Mg\n            <jats:sup>2+</jats:sup>\n            is the main charge carrier of the\n divalent current. This current is characterized by lanthanum\n sensitivity and a voltage-dependent blocking effect of\n Mg\n            <jats:sup>2+</jats:sup>\n            , which is relieved at both hyperpolarizing (inward\n rectification) and depolarizing (outward rectification) potentials. The\n store-operated divalent current is neither observed in native oocytes\n nor in oocytes expressing either TRP or TRPL alone. The production of\n this current implicates a cooperative action of TRP and TRPL in the\n depletion-activated current.\n          </jats:p>",
  "DOI": "10.1073/pnas.93.24.14146",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:43:20Z",
    "timestamp": 1027694600000
  },
  "page": "14146-14151",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 88,
  "title": "Coexpression of\n            <i>Drosophila</i>\n            TRP and TRP-like proteins in\n            <i>Xenopus</i>\n            oocytes reconstitutes capacitative Ca\n            <sup>2+</sup>\n            \u2009entry",
  "prefix": "10.1073",
  "volume": "93",
  "author": [
    {
      "given": "B.",
      "family": "Gillo",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    },
    {
      "given": "I.",
      "family": "Chorna",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    },
    {
      "given": "H.",
      "family": "Cohen",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    },
    {
      "given": "B.",
      "family": "Cook",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    },
    {
      "given": "I.",
      "family": "Manistersky",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    },
    {
      "given": "M.",
      "family": "Chorev",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    },
    {
      "given": "A.",
      "family": "Arnon",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    },
    {
      "given": "J.\u2009A.",
      "family": "Pollock",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    },
    {
      "given": "Z.",
      "family": "Selinger",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    },
    {
      "given": "B.",
      "family": "Minke",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Departments of Physiology, Pharmaceutical Chemistry,\r Biological Chemistry and the K\u00fchne Minerva\r Center for Studies of Visual Transduction, The Hebrew University,\r Jerusalem 91120, Israel; and Department of Biological Sciences,\r Carnegie Mellon University, Pittsburgh, PA 15213-3890"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1996,
        11,
        26
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_2_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0143-4160(90)90016-N"
    },
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/355353a0"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.24.14445"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.iy.13.040195.003203"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj3120001"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90408-5"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80132-4"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81021-1"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81233-7"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0143-4160(95)90023-3"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.ne.18.030195.001435"
    },
    {
      "key": "e_1_3_2_12_2",
      "first-page": "2340",
      "volume": "36",
      "author": "Pak W L",
      "year": "1995",
      "unstructured": "W L Pak Invest Ophthalmol Visual Sci 36, 2340\u20132357 (1995).",
      "journal-title": "Invest Ophthalmol Visual Sci"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-4388(96)80050-X"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0278-4327(91)90026-X"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/258084a0"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/224285a0"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1085/jgp.79.3.361"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00193462"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1085/jgp.98.4.849"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1098/rspb.1991.0110"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/354230a0"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(92)90086-S"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(94)90442-1"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.16-09-02924.1996"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1085/jgp.104.6.1057"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(89)90069-X"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(89)90117-7"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(92)90085-R"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81232-5"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/ajpcell.1994.267.5.C1501"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(94)01455-A"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.21.9652"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(95)01038-G"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80145-2"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/bbrc.1994.1808"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/ajpcell.1995.269.5.C1332"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj3110041"
    },
    {
      "key": "e_1_3_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.6.2955"
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(92)90239-A"
    },
    {
      "key": "e_1_3_2_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1994.tb06833.x"
    },
    {
      "key": "e_1_3_2_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.15-05-03747.1995"
    },
    {
      "key": "e_1_3_2_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1113/jphysiol.1987.sp016784"
    },
    {
      "key": "e_1_3_2_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00370215"
    },
    {
      "key": "e_1_3_2_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)31628-4"
    },
    {
      "key": "e_1_3_2_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1113/jphysiol.1993.sp019771"
    },
    {
      "key": "e_1_3_2_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/364814a0"
    },
    {
      "key": "e_1_3_2_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(94)90250-X"
    },
    {
      "key": "e_1_3_2_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/jn.1995.74.6.2590"
    },
    {
      "key": "e_1_3_2_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.1.1.99"
    },
    {
      "key": "e_1_3_2_50_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.13.6295"
    },
    {
      "key": "e_1_3_2_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0959-4388(93)90130-Q"
    },
    {
      "key": "e_1_3_2_52_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(94)80467-9"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.93.24.14146",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T19:02:27Z",
    "timestamp": 1649876547000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.93.24.14146"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1996,
        11,
        26
      ]
    ]
  },
  "references-count": 52,
  "journal-issue": {
    "issue": "24",
    "published-print": {
      "date-parts": [
        [
          1996,
          11,
          26
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.93.24.14146"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.93.24.14146",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1996,
        11,
        26
      ]
    ]
  },
  "assertion": [
    {
      "value": "1996-07-26",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1996-09-12",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "1996-11-26",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}