DOI: 10.1073/pnas.93.21.11288. Site-specific integration by adeno-associated virus.

Site-specific integration by adeno-associated virus.

10.1073/pnas.93.21.11288

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Adeno-associated virus (AAV) has attracted considerable interest as a potential vector for gene delivery. Wild-type virus is notable for the lack of association with any human disease and the ability to stably integrate its genome in a site-specific manner in a locus on human chromosome 19 (AAVS1). Use of a functional model system for AAV DNA integration into AAVS1 has allowed us to conclude that the recombination event is directed by cellular DNA sequences. Recombinant junctions isolated from our integration assay were analyzed and showed characteristics similar to those found in latently infected cell lines. The minimal DNA signals within AAVS1 required for targeted integration were identified and shown to contain functional motifs of the viral origin of replication. A replication mediated model of AAV DNA integration is proposed.

Bibliography

Linden, R. M., Ward, P., Giraud, C., Winocour, E., & Berns, K. I. (1996). Site-specific integration by adeno-associated virus. Proceedings of the National Academy of Sciences, 93(21), 11288â11294.

Authors 5

R M Linden (first)
P Ward (additional)
C Giraud (additional)
E Winocour (additional)
K I Berns (additional)

References 0 Referenced 236

None

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:35 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 3:27 p.m.)
Indexed	1 month, 1 week ago (July 25, 2025, 6:59 a.m.)
Issued	28 years, 10 months ago (Oct. 15, 1996)
Published	28 years, 10 months ago (Oct. 15, 1996)
Published Online	28 years, 10 months ago (Oct. 15, 1996)
Published Print	28 years, 10 months ago (Oct. 15, 1996)

Funders 0

None

BibTeX

@article{Linden_1996, title={Site-specific integration by adeno-associated virus.}, volume={93}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.93.21.11288}, DOI={10.1073/pnas.93.21.11288}, number={21}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Linden, R M and Ward, P and Giraud, C and Winocour, E and Berns, K I}, year={1996}, month=oct, pages={11288–11294} }

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