DOI: 10.1073/pnas.93.16.8253. A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix.

A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix.

10.1073/pnas.93.16.8253

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A hyperphosphorylated form of the largest subunit of RNA polymerase II (pol IIo) is associated with the pre-mRNA splicing process. Pol IIo was detected in association with a subset of small nuclear ribonucleoprotein particle and Ser-Arg protein splicing factors and also with pre-mRNA splicing complexes assembled in vitro. A subpopulation of pol IIo was localized to nuclear "speckle" domains enriched in splicing factors, indicating that it may also be associated with RNA processing in vivo. Moreover, pol IIo was retained in a similar pattern following in situ extraction of cells and was quantitatively recovered in the nuclear matrix fraction. The results implicate nuclear matrix-associated hyperphosphorylated pol IIo as a possible link in the coordination of transcription and splicing processes.

Bibliography

Mortillaro, M. J., Blencowe, B. J., Wei, X., Nakayasu, H., Du, L., Warren, S. L., Sharp, P. A., & Berezney, R. (1996). A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix. Proceedings of the National Academy of Sciences, 93(16), 8253â8257.

Authors 8

M J Mortillaro (first)
B J Blencowe (additional)
X Wei (additional)
H Nakayasu (additional)
L Du (additional)
S L Warren (additional)
P A Sharp (additional)
R Berezney (additional)

References 0 Referenced 251

None

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:39 p.m.)
Indexed	1 month, 2 weeks ago (July 2, 2025, 2:31 p.m.)
Issued	29 years ago (Aug. 6, 1996)
Published	29 years ago (Aug. 6, 1996)
Published Online	29 years ago (Aug. 6, 1996)
Published Print	29 years ago (Aug. 6, 1996)

Funders 0

None

BibTeX

@article{Mortillaro_1996, title={A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix.}, volume={93}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.93.16.8253}, DOI={10.1073/pnas.93.16.8253}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Mortillaro, M J and Blencowe, B J and Wei, X and Nakayasu, H and Du, L and Warren, S L and Sharp, P A and Berezney, R}, year={1996}, month=aug, pages={8253–8257} }

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