DOI: 10.1073/pnas.93.11.5395. Extremely sensitive, background-free gene detection using binary probes and beta replicase.

Extremely sensitive, background-free gene detection using binary probes and beta replicase.

10.1073/pnas.93.11.5395

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have developed a specific and sensitive nucleic acid amplification assay that is suitable for routine gene detection. The assay is based on a novel molecular genetic strategy in which two different RNA probes are hybridized to adjacent positions on a target nucleic acid and then ligated to form an amplifiable reporter RNA. The reporter RNA is then replicated up to a hundred billion-fold in a 30-min isothermal reaction that signals the presence of the target. The assay can detect fewer than 100 nucleic acid molecules; it provides quantitative results over a wide range of target concentrations and it employs a universal format that can detect any infectious agent.

Bibliography

Tyagi, S., Landegren, U., Tazi, M., Lizardi, P. M., & Kramer, F. R. (1996). Extremely sensitive, background-free gene detection using binary probes and beta replicase. Proceedings of the National Academy of Sciences, 93(11), 5395â5400.

Authors 5

S Tyagi (first)
U Landegren (additional)
M Tazi (additional)
P M Lizardi (additional)
F R Kramer (additional)

References 0 Referenced 45

None

Dates

Type	When
Created	23 years ago (July 26, 2002, 10:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2 p.m.)
Indexed	1 month, 1 week ago (July 11, 2025, 6:46 a.m.)
Issued	29 years, 2 months ago (May 28, 1996)
Published	29 years, 2 months ago (May 28, 1996)
Published Online	29 years, 2 months ago (May 28, 1996)
Published Print	29 years, 2 months ago (May 28, 1996)

Funders 0

None

BibTeX

@article{Tyagi_1996, title={Extremely sensitive, background-free gene detection using binary probes and beta replicase.}, volume={93}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.93.11.5395}, DOI={10.1073/pnas.93.11.5395}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Tyagi, S and Landegren, U and Tazi, M and Lizardi, P M and Kramer, F R}, year={1996}, month=may, pages={5395–5400} }

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