DOI: 10.1073/pnas.92.7.2451. Gas-phase folding and unfolding of cytochrome c cations.

Gas-phase folding and unfolding of cytochrome c cations.

10.1073/pnas.92.7.2451

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Water is thought to play a dominant role in protein folding, yet gaseous multiply protonated proteins from which the water has been completely removed show hydrogen/deuterium (H/D) exchange behavior similar to that used to identify conformations in solution. Indicative of the gas-phase accessibility to D2O, multiply-charged (6+ to 17+) cytochrome c cations exchange at six (or more) distinct levels of 64 to 173 out of 198 exchangeable H atoms, with the 132 H level found at charge values 8+ to 17+. Infrared laser heating and fast collisions can apparently induce ions to unfold to exchange at a higher distinct level, while charge-stripping ions to lower charge values yields apparent folding as well as unfolding.

Bibliography

Wood, T. D., Chorush, R. A., Wampler, F. M., Little, D. P., OâConnor, P. B., & McLafferty, F. W. (1995). Gas-phase folding and unfolding of cytochrome c cations. Proceedings of the National Academy of Sciences, 92(7), 2451â2454.

Authors 6

T D Wood (first)
R A Chorush (additional)
F M Wampler (additional)
D P Little (additional)
P B O'Connor (additional)
F W McLafferty (additional)

References 0 Referenced 205

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:29 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:07 p.m.)
Indexed	1 month, 2 weeks ago (July 2, 2025, 1:52 p.m.)
Issued	30 years, 4 months ago (March 28, 1995)
Published	30 years, 4 months ago (March 28, 1995)
Published Online	30 years, 4 months ago (March 28, 1995)
Published Print	30 years, 4 months ago (March 28, 1995)

Funders 0

None

BibTeX

@article{Wood_1995, title={Gas-phase folding and unfolding of cytochrome c cations.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.7.2451}, DOI={10.1073/pnas.92.7.2451}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wood, T D and Chorush, R A and Wampler, F M and Little, D P and O’Connor, P B and McLafferty, F W}, year={1995}, month=mar, pages={2451–2454} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T17:52:47Z",
    "timestamp": 1751478767555
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "7",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        3,
        28
      ]
    ]
  },
  "abstract": "<jats:p>Water is thought to play a dominant role in protein folding, yet gaseous multiply protonated proteins from which the water has been completely removed show hydrogen/deuterium (H/D) exchange behavior similar to that used to identify conformations in solution. Indicative of the gas-phase accessibility to D2O, multiply-charged (6+ to 17+) cytochrome c cations exchange at six (or more) distinct levels of 64 to 173 out of 198 exchangeable H atoms, with the 132 H level found at charge values 8+ to 17+. Infrared laser heating and fast collisions can apparently induce ions to unfold to exchange at a higher distinct level, while charge-stripping ions to lower charge values yields apparent folding as well as unfolding.</jats:p>",
  "DOI": "10.1073/pnas.92.7.2451",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T13:29:38Z",
    "timestamp": 1149082178000
  },
  "page": "2451-2454",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 205,
  "title": "Gas-phase folding and unfolding of cytochrome c cations.",
  "prefix": "10.1073",
  "volume": "92",
  "author": [
    {
      "given": "T D",
      "family": "Wood",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301, USA."
        }
      ]
    },
    {
      "given": "R A",
      "family": "Chorush",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301, USA."
        }
      ]
    },
    {
      "suffix": "3rd",
      "given": "F M",
      "family": "Wampler",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301, USA."
        }
      ]
    },
    {
      "given": "D P",
      "family": "Little",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301, USA."
        }
      ]
    },
    {
      "given": "P B",
      "family": "O'Connor",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301, USA."
        }
      ]
    },
    {
      "given": "F W",
      "family": "McLafferty",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301, USA."
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1995,
        3,
        28
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.92.7.2451",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T18:07:58Z",
    "timestamp": 1649873278000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.92.7.2451"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        3,
        28
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          1995,
          3,
          28
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.92.7.2451"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.92.7.2451",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1995,
        3,
        28
      ]
    ]
  },
  "assertion": [
    {
      "value": "1995-03-28",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}