DOI: 10.1073/pnas.92.6.1822. Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo.

Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo.

10.1073/pnas.92.6.1822

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are abundant RNA-binding proteins that are implicated in splicing regulation. Here we investigate the role of a Drosophila hnRNP in splicing regulation in living animals. We find that overexpression of the Drosophila hnRNP HRB98DE leads to skipping of all internal exons in the Drosophila dopa decarboxylase (Ddc) pre-mRNA in vivo. These results indicate that HRB98DE has a splicing activity that promotes use of terminal splice sites. The effect of excess HRB98DE on Ddc splicing is transient, even though high levels of HRB98DE persist for at least 24 hr. This suggests that Drosophila larvae can induce a compensating mechanism to counteract the effects of excess HRB98DE.

Bibliography

Shen, J., Zu, K., Cass, C. L., Beyer, A. L., & Hirsh, J. (1995). Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo. Proceedings of the National Academy of Sciences, 92(6), 1822â1825.

Authors 5

J Shen (first)
K Zu (additional)
C L Cass (additional)
A L Beyer (additional)
J Hirsh (additional)

References 0 Referenced 17

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:29 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:04 p.m.)
Indexed	1 year, 11 months ago (Sept. 13, 2023, 2:32 p.m.)
Issued	30 years, 5 months ago (March 14, 1995)
Published	30 years, 5 months ago (March 14, 1995)
Published Online	30 years, 5 months ago (March 14, 1995)
Published Print	30 years, 5 months ago (March 14, 1995)

Funders 0

None

BibTeX

@article{Shen_1995, title={Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.6.1822}, DOI={10.1073/pnas.92.6.1822}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Shen, J and Zu, K and Cass, C L and Beyer, A L and Hirsh, J}, year={1995}, month=mar, pages={1822–1825} }

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