DOI: 10.1073/pnas.92.26.12408. Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands.

Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands.

10.1073/pnas.92.26.12408

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Two dodecapeptides belonging to distinct classes of Src homology 3 (SH3) ligands and selected from biased phage display libraries were used to investigate interactions between a specificity pocket in the Src SH3 domain and ligant residues flanking the proline-rich core. The solution structures of c-Src SH3 complexed with these peptides were solved by NMR. In addition to proline-rich, polyproline type II helix-forming core, the class I and II ligands each possesses a flanking sequence that occupies a large pocket between the RT and n-Src loops of the SH3 domain. Structural and mutational analyses illustrate how the two classes of SH3 ligands exploit a specificity pocket on the receptor differently to increase binding affinity and specificity.

Bibliography

Feng, S., Kasahara, C., Rickles, R. J., & Schreiber, S. L. (1995). Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands. Proceedings of the National Academy of Sciences, 92(26), 12408â12415.

Authors 4

S Feng (first)
C Kasahara (additional)
R J Rickles (additional)
S L Schreiber (additional)

References 0 Referenced 196

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 9:26 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:02 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 6, 2025, 9:54 a.m.)
Issued	29 years, 8 months ago (Dec. 19, 1995)
Published	29 years, 8 months ago (Dec. 19, 1995)
Published Online	29 years, 8 months ago (Dec. 19, 1995)
Published Print	29 years, 8 months ago (Dec. 19, 1995)

Funders 0

None

BibTeX

@article{Feng_1995, title={Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.26.12408}, DOI={10.1073/pnas.92.26.12408}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Feng, S and Kasahara, C and Rickles, R J and Schreiber, S L}, year={1995}, month=dec, pages={12408–12415} }

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