DOI: 10.1073/pnas.92.25.11618. Cloning and characterization of Lnk, a signal transduction protein that links T-cell receptor activation signal to phospholipase C gamma 1, Grb2, and phosphatidylinositol 3-kinase.

Cloning and characterization of Lnk, a signal transduction protein that links T-cell receptor activation signal to phospholipase C gamma 1, Grb2, and phosphatidylinositol 3-kinase.

10.1073/pnas.92.25.11618

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A cDNA encoding a signal transduction protein with a Src homology 2 (SH2) domain and a tyrosine phosphorylation site was cloned from a rat lymph node cDNA library. This protein, which we designate Lnk, has a calculated molecular weight of 33,988. When T lymphocytes were activated by antibody-mediated crosslinking of the T-cell receptor and CD4, Lnk became tyrosine phosphorylated. In activated T lymphocytes, phospholipase C gamma 1, phosphatidylinositol 3-kinase, and Grb-2 coimmunoprecipitated with Lnk. Our results suggest that Lnk becomes tyrosine phosphorylated and links the immediate tyrosine phosphorylation signals of the TCR to the distal phosphatidylinositol 3-kinase, phospholipase C gamma 1 and Ras signaling pathways through its multifunctional tyrosine phosphorylation site.

Bibliography

Huang, X., Li, Y., Tanaka, K., Moore, K. G., & Hayashi, J. I. (1995). Cloning and characterization of Lnk, a signal transduction protein that links T-cell receptor activation signal to phospholipase C gamma 1, Grb2, and phosphatidylinositol 3-kinase. Proceedings of the National Academy of Sciences, 92(25), 11618â11622.

Authors 5

X Huang (first)
Y Li (additional)
K Tanaka (additional)
K G Moore (additional)
J I Hayashi (additional)

References 0 Referenced 127

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:24 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:58 p.m.)
Indexed	4 months, 3 weeks ago (March 31, 2025, 8:43 a.m.)
Issued	29 years, 8 months ago (Dec. 5, 1995)
Published	29 years, 8 months ago (Dec. 5, 1995)
Published Online	29 years, 8 months ago (Dec. 5, 1995)
Published Print	29 years, 8 months ago (Dec. 5, 1995)

Funders 0

None

BibTeX

@article{Huang_1995, title={Cloning and characterization of Lnk, a signal transduction protein that links T-cell receptor activation signal to phospholipase C gamma 1, Grb2, and phosphatidylinositol 3-kinase.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.25.11618}, DOI={10.1073/pnas.92.25.11618}, number={25}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Huang, X and Li, Y and Tanaka, K and Moore, K G and Hayashi, J I}, year={1995}, month=dec, pages={11618–11622} }

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