DOI: 10.1073/pnas.92.19.8719. Ligand-induced endocytosis of epidermal growth factor receptors that are defective in binding adaptor proteins.

Ligand-induced endocytosis of epidermal growth factor receptors that are defective in binding adaptor proteins.

10.1073/pnas.92.19.8719

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Ligand-activated epidermal growth factor receptors (EGFRs) associate with coated pit adaptor proteins (AP2) in vivo, implying a mechanism for receptor retention in coated pits during internalization. Using an in vitro binding assay, we localized the adaptor binding determinant to residues 970-991 of EGFRs and confirmed specificity by competition with a synthetic peptide corresponding to this sequence. A mutant EGFR lacking this AP2 binding determinant did not associate with AP2 in vivo but demonstrated internalization and down-regulation kinetics indistinguishable from its wild-type counterpart. Immunocytochemistry confirmed ligand-induced internalization of the mutant EGFR. These data suggest that endocytic determinants are distinct from AP2 binding determinants and that processes other than association with AP2 regulate endocytosis of EGFRs.

Bibliography

Nesterov, A., Wiley, H. S., & Gill, G. N. (1995). Ligand-induced endocytosis of epidermal growth factor receptors that are defective in binding adaptor proteins. Proceedings of the National Academy of Sciences, 92(19), 8719â8723.

Authors 3

A Nesterov (first)
H S Wiley (additional)
G N Gill (additional)

References 0 Referenced 69

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:19 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:58 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:32 p.m.)
Issued	29 years, 11 months ago (Sept. 12, 1995)
Published	29 years, 11 months ago (Sept. 12, 1995)
Published Online	29 years, 11 months ago (Sept. 12, 1995)
Published Print	29 years, 11 months ago (Sept. 12, 1995)

Funders 0

None

BibTeX

@article{Nesterov_1995, title={Ligand-induced endocytosis of epidermal growth factor receptors that are defective in binding adaptor proteins.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.19.8719}, DOI={10.1073/pnas.92.19.8719}, number={19}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Nesterov, A and Wiley, H S and Gill, G N}, year={1995}, month=sep, pages={8719–8723} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T18:32:46Z",
    "timestamp": 1751481166181
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "19",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        9,
        12
      ]
    ]
  },
  "abstract": "<jats:p>Ligand-activated epidermal growth factor receptors (EGFRs) associate with coated pit adaptor proteins (AP2) in vivo, implying a mechanism for receptor retention in coated pits during internalization. Using an in vitro binding assay, we localized the adaptor binding determinant to residues 970-991 of EGFRs and confirmed specificity by competition with a synthetic peptide corresponding to this sequence. A mutant EGFR lacking this AP2 binding determinant did not associate with AP2 in vivo but demonstrated internalization and down-regulation kinetics indistinguishable from its wild-type counterpart. Immunocytochemistry confirmed ligand-induced internalization of the mutant EGFR. These data suggest that endocytic determinants are distinct from AP2 binding determinants and that processes other than association with AP2 regulate endocytosis of EGFRs.</jats:p>",
  "DOI": "10.1073/pnas.92.19.8719",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T13:19:27Z",
    "timestamp": 1149081567000
  },
  "page": "8719-8723",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 69,
  "title": "Ligand-induced endocytosis of epidermal growth factor receptors that are defective in binding adaptor proteins.",
  "prefix": "10.1073",
  "volume": "92",
  "author": [
    {
      "given": "A",
      "family": "Nesterov",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Medicine, University of California, San Diego School of Medicine, La Jolla 92093, USA."
        }
      ]
    },
    {
      "given": "H S",
      "family": "Wiley",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Medicine, University of California, San Diego School of Medicine, La Jolla 92093, USA."
        }
      ]
    },
    {
      "given": "G N",
      "family": "Gill",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Medicine, University of California, San Diego School of Medicine, La Jolla 92093, USA."
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1995,
        9,
        12
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.92.19.8719",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T17:58:58Z",
    "timestamp": 1649872738000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.92.19.8719"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        9,
        12
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "19",
    "published-print": {
      "date-parts": [
        [
          1995,
          9,
          12
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.92.19.8719"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.92.19.8719",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1995,
        9,
        12
      ]
    ]
  },
  "assertion": [
    {
      "value": "1995-09-12",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}