DOI: 10.1073/pnas.92.15.7026. Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.

Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.

10.1073/pnas.92.15.7026

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Transcription factor TFIIIB plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. We report the purification of a human TFIIIB-derived complex containing only the TATA-binding polypeptide (TBP) and a 90-kDa subunit (TFIIIB90) and the isolation of a cDNA clone encoding the 90-kDa subunit. The N-terminal half of TFIIIB90 exhibits sequence similarity to the yeast TFIIIB70 (BRF) and the class II transcription factor TFIIB and interacts weakly with TBP. The C-terminal half of TFIIIB90 contains a high-mobility-group protein 2 (HMG2)-related domain and interacts strongly with TBP. Recombinant TFIIIB90 plus recombinant human TBP substitute for human TFIIIB in a complementation assay for transcription of 5S, tRNA, and VA1 RNA genes, and both the TFIIB-related domain and the HMG2-related domain are required for this activity. TFIIIB90 is also required for transcription of human 7SK and U6 RNA genes by RNA polymerase III, but apparently within a complex distinct from the TBP/TFIIIB90 complex.

Bibliography

Wang, Z., & Roeder, R. G. (1995). Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains. Proceedings of the National Academy of Sciences, 92(15), 7026â7030.

Authors 2

Z Wang (first)
R G Roeder (additional)

References 0 Referenced 98

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:16 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:50 p.m.)
Indexed	3 months, 1 week ago (May 15, 2025, 1:10 p.m.)
Issued	30 years, 1 month ago (July 18, 1995)
Published	30 years, 1 month ago (July 18, 1995)
Published Online	30 years, 1 month ago (July 18, 1995)
Published Print	30 years, 1 month ago (July 18, 1995)

Funders 0

None

BibTeX

@article{Wang_1995, title={Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.15.7026}, DOI={10.1073/pnas.92.15.7026}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wang, Z and Roeder, R G}, year={1995}, month=jul, pages={7026–7030} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        15
      ]
    ],
    "date-time": "2025-05-15T17:10:10Z",
    "timestamp": 1747329010945
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "15",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        7,
        18
      ]
    ]
  },
  "abstract": "<jats:p>Transcription factor TFIIIB plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. We report the purification of a human TFIIIB-derived complex containing only the TATA-binding polypeptide (TBP) and a 90-kDa subunit (TFIIIB90) and the isolation of a cDNA clone encoding the 90-kDa subunit. The N-terminal half of TFIIIB90 exhibits sequence similarity to the yeast TFIIIB70 (BRF) and the class II transcription factor TFIIB and interacts weakly with TBP. The C-terminal half of TFIIIB90 contains a high-mobility-group protein 2 (HMG2)-related domain and interacts strongly with TBP. Recombinant TFIIIB90 plus recombinant human TBP substitute for human TFIIIB in a complementation assay for transcription of 5S, tRNA, and VA1 RNA genes, and both the TFIIB-related domain and the HMG2-related domain are required for this activity. TFIIIB90 is also required for transcription of human 7SK and U6 RNA genes by RNA polymerase III, but apparently within a complex distinct from the TBP/TFIIIB90 complex.</jats:p>",
  "DOI": "10.1073/pnas.92.15.7026",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T13:16:00Z",
    "timestamp": 1149081360000
  },
  "page": "7026-7030",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 98,
  "title": "Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.",
  "prefix": "10.1073",
  "volume": "92",
  "author": [
    {
      "given": "Z",
      "family": "Wang",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Rockefeller University, New York, NY 10021, USA."
        }
      ]
    },
    {
      "given": "R G",
      "family": "Roeder",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Rockefeller University, New York, NY 10021, USA."
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1995,
        7,
        18
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.92.15.7026",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T17:50:23Z",
    "timestamp": 1649872223000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.92.15.7026"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        7,
        18
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "15",
    "published-print": {
      "date-parts": [
        [
          1995,
          7,
          18
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.92.15.7026"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.92.15.7026",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1995,
        7,
        18
      ]
    ]
  },
  "assertion": [
    {
      "value": "1995-07-18",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}