DOI: 10.1073/pnas.92.14.6592. Capturing genes encoding membrane and secreted proteins important for mouse development.

Capturing genes encoding membrane and secreted proteins important for mouse development.

10.1073/pnas.92.14.6592

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A strategy based on the gene trap was developed to prescreen mouse embryonic stem cells for insertional mutations in genes encoding secreted and membrane-spanning proteins. The "secretory trap" relies on capturing the N-terminal signal sequence of an endogenous gene to generate an active beta-galactosidase fusion protein. Insertions were found in a cadherin gene, an unc6-related laminin (netrin) gene, the sek receptor tyrosine kinase gene, and genes encoding two receptor-linked protein-tyrosine phosphatases, LAR and PTP kappa. Analysis of homozygous mice carrying insertions in LAR and PTP kappa showed that both genes were effectively disrupted, but neither was essential for normal embryonic development.

Bibliography

Skarnes, W. C., Moss, J. E., Hurtley, S. M., & Beddington, R. S. (1995). Capturing genes encoding membrane and secreted proteins important for mouse development. Proceedings of the National Academy of Sciences, 92(14), 6592â6596.

Authors 4

W C Skarnes (first)
J E Moss (additional)
S M Hurtley (additional)
R S Beddington (additional)

References 0 Referenced 257

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:14 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:02 p.m.)
Indexed	2 weeks, 5 days ago (Aug. 7, 2025, 4:55 a.m.)
Issued	30 years, 1 month ago (July 3, 1995)
Published	30 years, 1 month ago (July 3, 1995)
Published Online	30 years, 1 month ago (July 3, 1995)
Published Print	30 years, 1 month ago (July 3, 1995)

Funders 0

None

BibTeX

@article{Skarnes_1995, title={Capturing genes encoding membrane and secreted proteins important for mouse development.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.14.6592}, DOI={10.1073/pnas.92.14.6592}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Skarnes, W C and Moss, J E and Hurtley, S M and Beddington, R S}, year={1995}, month=jul, pages={6592–6596} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T08:55:14Z",
    "timestamp": 1754556914384
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "14",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        7,
        3
      ]
    ]
  },
  "abstract": "<jats:p>A strategy based on the gene trap was developed to prescreen mouse embryonic stem cells for insertional mutations in genes encoding secreted and membrane-spanning proteins. The \"secretory trap\" relies on capturing the N-terminal signal sequence of an endogenous gene to generate an active beta-galactosidase fusion protein. Insertions were found in a cadherin gene, an unc6-related laminin (netrin) gene, the sek receptor tyrosine kinase gene, and genes encoding two receptor-linked protein-tyrosine phosphatases, LAR and PTP kappa. Analysis of homozygous mice carrying insertions in LAR and PTP kappa showed that both genes were effectively disrupted, but neither was essential for normal embryonic development.</jats:p>",
  "DOI": "10.1073/pnas.92.14.6592",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T13:14:14Z",
    "timestamp": 1149081254000
  },
  "page": "6592-6596",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 257,
  "title": "Capturing genes encoding membrane and secreted proteins important for mouse development.",
  "prefix": "10.1073",
  "volume": "92",
  "author": [
    {
      "given": "W C",
      "family": "Skarnes",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Biotechnology and Biological Sciences Research Council, Centre for Genome Research, University of Edinburgh, United Kingdom."
        }
      ]
    },
    {
      "given": "J E",
      "family": "Moss",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Biotechnology and Biological Sciences Research Council, Centre for Genome Research, University of Edinburgh, United Kingdom."
        }
      ]
    },
    {
      "given": "S M",
      "family": "Hurtley",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Biotechnology and Biological Sciences Research Council, Centre for Genome Research, University of Edinburgh, United Kingdom."
        }
      ]
    },
    {
      "given": "R S",
      "family": "Beddington",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Biotechnology and Biological Sciences Research Council, Centre for Genome Research, University of Edinburgh, United Kingdom."
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1995,
        7,
        3
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.92.14.6592",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T18:02:22Z",
    "timestamp": 1649872942000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.92.14.6592"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        7,
        3
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "14",
    "published-print": {
      "date-parts": [
        [
          1995,
          7,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.92.14.6592"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.92.14.6592",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1995,
        7,
        3
      ]
    ]
  },
  "assertion": [
    {
      "value": "1995-07-03",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}