DOI: 10.1073/pnas.92.13.5940. Ligand-regulated site-specific recombination.

Ligand-regulated site-specific recombination.

10.1073/pnas.92.13.5940

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Site-specific recombination offers a potential way to alter a living genome by design in a precise and stable manner. This potential requires strategies which can be used to regulate the recombination event. We describe a strategy to regulate FLP recombinase activity which relies on expressing FLP as a fusion protein with steroid hormone receptor ligand binding domains (LBDs). In the absence of a ligand cognate to the LBD, the recombinase activity of the fusion protein is extremely low. Upon ligand administration, recombinase activity is rapidly induced. These results outline the basis for inducible expression or disruption strategies based on inducible recombination. Additionally, we have exploited the conditional nature of FLP-LBD fusion proteins to direct integration of a plasmid into a specific genomic site at frequencies approaching the frequency of random integration.

Bibliography

Logie, C., & Stewart, A. F. (1995). Ligand-regulated site-specific recombination. Proceedings of the National Academy of Sciences, 92(13), 5940â5944.

Authors 2

C Logie (first)
A F Stewart (additional)

References 0 Referenced 133

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:13 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:03 p.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 9:31 a.m.)
Issued	30 years, 2 months ago (June 20, 1995)
Published	30 years, 2 months ago (June 20, 1995)
Published Online	30 years, 2 months ago (June 20, 1995)
Published Print	30 years, 2 months ago (June 20, 1995)

Funders 0

None

BibTeX

@article{Logie_1995, title={Ligand-regulated site-specific recombination.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.13.5940}, DOI={10.1073/pnas.92.13.5940}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Logie, C and Stewart, A F}, year={1995}, month=jun, pages={5940–5944} }

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