DOI: 10.1073/pnas.92.10.4572. Plasmid DNA entry into postmitotic nuclei of primary rat myotubes.

Plasmid DNA entry into postmitotic nuclei of primary rat myotubes.

10.1073/pnas.92.10.4572

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

These studies were initiated to elucidate the mechanism of DNA nuclear transport in mammalian cells. Biotin- or gold-labeled plasmid and plasmid DNA expression vectors for Escherichia coli beta-galactosidase or firefly luciferase were microinjected into the cytoplasm of primary rat myotubes in culture. Plasmid DNA was expressed in up to 70% of the injected myotubes, which indicates that it entered intact, postmitotic nuclei. The nuclear transport of plasmid DNA occurred through the nuclear pore by a process common to other large karyophilic macromolecules. The majority of the injected plasmid DNA was sequestered by cytoplasmic elements. This understanding of plasmid DNA nuclear transport provides a basis for increasing the efficiency of gene transfer.

Bibliography

Dowty, M. E., Williams, P., Zhang, G., Hagstrom, J. E., & Wolff, J. A. (1995). Plasmid DNA entry into postmitotic nuclei of primary rat myotubes. Proceedings of the National Academy of Sciences, 92(10), 4572â4576.

Authors 5

M E Dowty (first)
P Williams (additional)
G Zhang (additional)
J E Hagstrom (additional)
J A Wolff (additional)

References 0 Referenced 218

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:09 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:57 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 2, 2025, 1:25 a.m.)
Issued	30 years, 3 months ago (May 9, 1995)
Published	30 years, 3 months ago (May 9, 1995)
Published Online	30 years, 3 months ago (May 9, 1995)
Published Print	30 years, 3 months ago (May 9, 1995)

Funders 0

None

BibTeX

@article{Dowty_1995, title={Plasmid DNA entry into postmitotic nuclei of primary rat myotubes.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.10.4572}, DOI={10.1073/pnas.92.10.4572}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Dowty, M E and Williams, P and Zhang, G and Hagstrom, J E and Wolff, J A}, year={1995}, month=may, pages={4572–4576} }

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