DOI: 10.1073/pnas.92.10.4352. Exit from G0 and entry into the cell cycle of cells expressing p21Sdi1 antisense RNA.

Exit from G0 and entry into the cell cycle of cells expressing p21Sdi1 antisense RNA.

10.1073/pnas.92.10.4352

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

p21Sdi1 (also known as Cip1 and Waf1), an inhibitor of DNA synthesis cloned from senescent human fibroblasts, is an inhibitor of G1 cyclin-dependent kinases (Cdks) in vitro and is transcriptionally regulated by wild-type p53. In addition, p21Sdi1 has been found to inhibit DNA replication by direct interaction with proliferating cell nuclear antigen. In this study we analyzed normal human fibroblast cells arrested in G0 and determined that an excess of p21Sdi1 was present after immunodepletion of various cyclins and Cdks, in contrast to mitogen-stimulated cells in early S phase. Expression of antisense p21Sdi1 RNA in G0-arrested cells resulted in induction of DNA synthesis as well as entry into mitosis. These results suggest that p21Sdi1 functions in G0 and early G1 and that decreased expression of the gene is necessary for cell cycle progression.

Bibliography

Nakanishi, M., Adami, G. R., Robetorye, R. S., Noda, A., Venable, S. F., Dimitrov, D., Pereira-Smith, O. M., & Smith, J. R. (1995). Exit from G0 and entry into the cell cycle of cells expressing p21Sdi1 antisense RNA. Proceedings of the National Academy of Sciences, 92(10), 4352â4356.

Authors 8

M Nakanishi (first)
G R Adami (additional)
R S Robetorye (additional)
A Noda (additional)
S F Venable (additional)
D Dimitrov (additional)
O M Pereira-Smith (additional)
J R Smith (additional)

References 0 Referenced 101

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:09 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:55 p.m.)
Indexed	2 days, 19 hours ago (Aug. 20, 2025, 8:28 a.m.)
Issued	30 years, 3 months ago (May 9, 1995)
Published	30 years, 3 months ago (May 9, 1995)
Published Online	30 years, 3 months ago (May 9, 1995)
Published Print	30 years, 3 months ago (May 9, 1995)

Funders 0

None

BibTeX

@article{Nakanishi_1995, title={Exit from G0 and entry into the cell cycle of cells expressing p21Sdi1 antisense RNA.}, volume={92}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.92.10.4352}, DOI={10.1073/pnas.92.10.4352}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Nakanishi, M and Adami, G R and Robetorye, R S and Noda, A and Venable, S F and Dimitrov, D and Pereira-Smith, O M and Smith, J R}, year={1995}, month=may, pages={4352–4356} }

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