DOI: 10.1073/pnas.91.9.4014. Purification and physiological evaluation of a guanylate cyclase activating protein from retinal rods.

Purification and physiological evaluation of a guanylate cyclase activating protein from retinal rods.

10.1073/pnas.91.9.4014

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In retinal rods light triggers a cascade of enzymatic reactions that increases cGMP hydrolysis and generates an electrical signal by causing closure of cGMP-gated ion channels in the photoreceptor outer segment. This leads to a decrease in internal Ca, which activates guanylate cyclase and promotes photoresponse recovery by stimulating the resynthesis of cGMP. We report here that the activation of guanylate cyclase by low Ca is mediated by an approximately 20-kDa protein purified from bovine rod outer segments by using DEAE-Sepharose, hydroxylapatite, and reverse-phase chromatographies. In a reconstituted system, this protein restores the Ca-sensitive regulation of guanylate cyclase and when dialyzed into functionally intact lizard rod outer segment decreases the sensitivity, time to peak, and recovery time of the flash response.

Bibliography

Gorczyca, W. A., Gray-Keller, M. P., Detwiler, P. B., & Palczewski, K. (1994). Purification and physiological evaluation of a guanylate cyclase activating protein from retinal rods. Proceedings of the National Academy of Sciences, 91(9), 4014â4018.

Authors 4

W A Gorczyca (first)
M P Gray-Keller (additional)
P B Detwiler (additional)
K Palczewski (additional)

References 0 Referenced 190

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 9:09 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:11 p.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 10:30 a.m.)
Issued	31 years, 4 months ago (April 26, 1994)
Published	31 years, 4 months ago (April 26, 1994)
Published Online	31 years, 4 months ago (April 26, 1994)
Published Print	31 years, 4 months ago (April 26, 1994)

Funders 0

None

BibTeX

@article{Gorczyca_1994, title={Purification and physiological evaluation of a guanylate cyclase activating protein from retinal rods.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.9.4014}, DOI={10.1073/pnas.91.9.4014}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gorczyca, W A and Gray-Keller, M P and Detwiler, P B and Palczewski, K}, year={1994}, month=apr, pages={4014–4018} }

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