DOI: 10.1073/pnas.91.8.2905. Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement.

Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement.

10.1073/pnas.91.8.2905

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The Saccharomyces cerevisiae SWI1, SWI2 (SNF2), SWI3, SNF5, and SNF6 gene products play a crucial role in the regulation of transcription. We provide here direct biochemical evidence that all five SWI/SNF polypeptides are components of a large multisubunit complex. These five polypeptides coelute from a gel-filtration column with an apparent molecular mass of approximately 2 MDa. The five SWI/SNF polypeptides do not copurify when extracts are prepared from swi- or snf- mutants. We show that SWI/SNF polypeptides also remain associated during an affinity-chromatography step followed by gel filtration. Assembly of the SWI/SNF complex is not disrupted by a mutation in the putative APT-binding site of SWI2, although this mutation eliminates SWI2 function.

Bibliography

Peterson, C. L., Dingwall, A., & Scott, M. P. (1994). Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement. Proceedings of the National Academy of Sciences, 91(8), 2905â2908.

Authors 3

C L Peterson (first)
A Dingwall (additional)
M P Scott (additional)

References 0 Referenced 305

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 9:08 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:14 p.m.)
Indexed	1 month ago (Aug. 5, 2025, 8:41 a.m.)
Issued	31 years, 4 months ago (April 12, 1994)
Published	31 years, 4 months ago (April 12, 1994)
Published Online	31 years, 4 months ago (April 12, 1994)
Published Print	31 years, 4 months ago (April 12, 1994)

Funders 0

None

BibTeX

@article{Peterson_1994, title={Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.8.2905}, DOI={10.1073/pnas.91.8.2905}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Peterson, C L and Dingwall, A and Scott, M P}, year={1994}, month=apr, pages={2905–2908} }

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