DOI: 10.1073/pnas.91.7.2723. Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins.

Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins.

10.1073/pnas.91.7.2723

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Proteins secreted by the yeast Saccharomyces cerevisiae are usually modified by the addition at asparagine-linked glycosylation sites of large heterogeneous mannan units that are highly immunogenic. Secreted proteins from mnn1 mnn9 mutant strains, in contrast, have homogeneous Man10GlcNAc2 oligosaccharides that lack the immunogenic alpha 1,3-mannose linkages. We have cloned and sequenced the MNN9 and MNN1 genes, both of which encode proteins with the characteristics of type II membrane proteins. Mnn9p is a membrane-associated protein with unknown function that is required for the addition of the long alpha 1,6-mannose backbone of the complex mannan, whereas Mnn1p is most likely the alpha 1,3-mannosyltransferase located in the Golgi apparatus.

Bibliography

Yip, C. L., Welch, S. K., Klebl, F., Gilbert, T., Seidel, P., Grant, F. J., OâHara, P. J., & MacKay, V. L. (1994). Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins. Proceedings of the National Academy of Sciences, 91(7), 2723â2727.

Authors 8

C L Yip (first)
S K Welch (additional)
F Klebl (additional)
T Gilbert (additional)
P Seidel (additional)
F J Grant (additional)
P J O'Hara (additional)
V L MacKay (additional)

References 0 Referenced 105

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 9:06 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:51 p.m.)
Indexed	2 months, 1 week ago (June 23, 2025, 11:24 a.m.)
Issued	31 years, 5 months ago (March 29, 1994)
Published	31 years, 5 months ago (March 29, 1994)
Published Online	31 years, 5 months ago (March 29, 1994)
Published Print	31 years, 5 months ago (March 29, 1994)

Funders 0

None

BibTeX

@article{Yip_1994, title={Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.7.2723}, DOI={10.1073/pnas.91.7.2723}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yip, C L and Welch, S K and Klebl, F and Gilbert, T and Seidel, P and Grant, F J and O’Hara, P J and MacKay, V L}, year={1994}, month=mar, pages={2723–2727} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        23
      ]
    ],
    "date-time": "2025-06-23T15:24:57Z",
    "timestamp": 1750692297079
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "7",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1994,
        3,
        29
      ]
    ]
  },
  "abstract": "<jats:p>Proteins secreted by the yeast Saccharomyces cerevisiae are usually modified by the addition at asparagine-linked glycosylation sites of large heterogeneous mannan units that are highly immunogenic. Secreted proteins from mnn1 mnn9 mutant strains, in contrast, have homogeneous Man10GlcNAc2 oligosaccharides that lack the immunogenic alpha 1,3-mannose linkages. We have cloned and sequenced the MNN9 and MNN1 genes, both of which encode proteins with the characteristics of type II membrane proteins. Mnn9p is a membrane-associated protein with unknown function that is required for the addition of the long alpha 1,6-mannose backbone of the complex mannan, whereas Mnn1p is most likely the alpha 1,3-mannosyltransferase located in the Golgi apparatus.</jats:p>",
  "DOI": "10.1073/pnas.91.7.2723",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T13:06:33Z",
    "timestamp": 1149080793000
  },
  "page": "2723-2727",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 105,
  "title": "Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins.",
  "prefix": "10.1073",
  "volume": "91",
  "author": [
    {
      "given": "C L",
      "family": "Yip",
      "sequence": "first",
      "affiliation": [
        {
          "name": "ZymoGenetics, Inc., Seattle, WA 98105."
        }
      ]
    },
    {
      "given": "S K",
      "family": "Welch",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "ZymoGenetics, Inc., Seattle, WA 98105."
        }
      ]
    },
    {
      "given": "F",
      "family": "Klebl",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "ZymoGenetics, Inc., Seattle, WA 98105."
        }
      ]
    },
    {
      "given": "T",
      "family": "Gilbert",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "ZymoGenetics, Inc., Seattle, WA 98105."
        }
      ]
    },
    {
      "given": "P",
      "family": "Seidel",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "ZymoGenetics, Inc., Seattle, WA 98105."
        }
      ]
    },
    {
      "given": "F J",
      "family": "Grant",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "ZymoGenetics, Inc., Seattle, WA 98105."
        }
      ]
    },
    {
      "given": "P J",
      "family": "O'Hara",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "ZymoGenetics, Inc., Seattle, WA 98105."
        }
      ]
    },
    {
      "given": "V L",
      "family": "MacKay",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "ZymoGenetics, Inc., Seattle, WA 98105."
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1994,
        3,
        29
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.91.7.2723",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T17:51:10Z",
    "timestamp": 1649872270000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.91.7.2723"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1994,
        3,
        29
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          1994,
          3,
          29
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.91.7.2723"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.91.7.2723",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1994,
        3,
        29
      ]
    ]
  },
  "assertion": [
    {
      "value": "1994-03-29",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}