DOI: 10.1073/pnas.91.6.2270. Biochemical evidence for a complex involving dihydropyridine receptor and ryanodine receptor in triad junctions of skeletal muscle.

Biochemical evidence for a complex involving dihydropyridine receptor and ryanodine receptor in triad junctions of skeletal muscle.

10.1073/pnas.91.6.2270

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Membrane vesicles enriched in both ryanodine receptor and dihydropyridine receptor were obtained from rabbit skeletal muscle and solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Analysis of the sedimentation behavior of the solubilized proteins showed the existence of a population of alpha 1 subunits of the dihydropyridine receptor which cosedimented with the ryanodine receptor. Solubilized proteins were immunoprecipitated with antibodies directed against either the ryanodine receptor or the alpha 1, alpha 2, or beta subunits of the dihydropyridine receptor. Immunoprecipitated proteins were identified by Western blot analysis and by specific labeling with [3H]ryanodine or [3H]PN200-110. Immunoprecipitation of the solubilized proteins with antibodies directed against the dihydropyridine receptor led to the coimmunoprecipitation of the ryanodine receptor. Conversely, immunoprecipitation with antibodies directed against the ryanodine receptor led to an immune complex containing both receptors, but these antibodies were unable to precipitate purified dihydropyridine receptor. These results demonstrate that ryanodine receptor and dihydropyridine receptor are present in the triad membrane preparation in a complex which may play an important role in excitation-contraction coupling.

Bibliography

Marty, I., Robert, M., Villaz, M., De Jongh, K., Lai, Y., Catterall, W. A., & Ronjat, M. (1994). Biochemical evidence for a complex involving dihydropyridine receptor and ryanodine receptor in triad junctions of skeletal muscle. Proceedings of the National Academy of Sciences, 91(6), 2270â2274.

Authors 7

I Marty (first)
M Robert (additional)
M Villaz (additional)
K De Jongh (additional)
Y Lai (additional)
W A Catterall (additional)
M Ronjat (additional)

References 0 Referenced 118

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:05 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:49 p.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 7:30 a.m.)
Issued	31 years, 5 months ago (March 15, 1994)
Published	31 years, 5 months ago (March 15, 1994)
Published Online	31 years, 5 months ago (March 15, 1994)
Published Print	31 years, 5 months ago (March 15, 1994)

Funders 0

None

BibTeX

@article{Marty_1994, title={Biochemical evidence for a complex involving dihydropyridine receptor and ryanodine receptor in triad junctions of skeletal muscle.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.6.2270}, DOI={10.1073/pnas.91.6.2270}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Marty, I and Robert, M and Villaz, M and De Jongh, K and Lai, Y and Catterall, W A and Ronjat, M}, year={1994}, month=mar, pages={2270–2274} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        8
      ]
    ],
    "date-time": "2025-07-08T11:30:34Z",
    "timestamp": 1751974234015
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "6",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1994,
        3,
        15
      ]
    ]
  },
  "abstract": "<jats:p>Membrane vesicles enriched in both ryanodine receptor and dihydropyridine receptor were obtained from rabbit skeletal muscle and solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Analysis of the sedimentation behavior of the solubilized proteins showed the existence of a population of alpha 1 subunits of the dihydropyridine receptor which cosedimented with the ryanodine receptor. Solubilized proteins were immunoprecipitated with antibodies directed against either the ryanodine receptor or the alpha 1, alpha 2, or beta subunits of the dihydropyridine receptor. Immunoprecipitated proteins were identified by Western blot analysis and by specific labeling with [3H]ryanodine or [3H]PN200-110. Immunoprecipitation of the solubilized proteins with antibodies directed against the dihydropyridine receptor led to the coimmunoprecipitation of the ryanodine receptor. Conversely, immunoprecipitation with antibodies directed against the ryanodine receptor led to an immune complex containing both receptors, but these antibodies were unable to precipitate purified dihydropyridine receptor. These results demonstrate that ryanodine receptor and dihydropyridine receptor are present in the triad membrane preparation in a complex which may play an important role in excitation-contraction coupling.</jats:p>",
  "DOI": "10.1073/pnas.91.6.2270",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T13:05:57Z",
    "timestamp": 1149080757000
  },
  "page": "2270-2274",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 118,
  "title": "Biochemical evidence for a complex involving dihydropyridine receptor and ryanodine receptor in triad junctions of skeletal muscle.",
  "prefix": "10.1073",
  "volume": "91",
  "author": [
    {
      "given": "I",
      "family": "Marty",
      "sequence": "first",
      "affiliation": [
        {
          "name": "D\u00e9partement de Biologie Mol\u00e9culaire et Structurale, CENG 85X, Grenoble, France."
        }
      ]
    },
    {
      "given": "M",
      "family": "Robert",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "D\u00e9partement de Biologie Mol\u00e9culaire et Structurale, CENG 85X, Grenoble, France."
        }
      ]
    },
    {
      "given": "M",
      "family": "Villaz",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "D\u00e9partement de Biologie Mol\u00e9culaire et Structurale, CENG 85X, Grenoble, France."
        }
      ]
    },
    {
      "given": "K",
      "family": "De Jongh",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "D\u00e9partement de Biologie Mol\u00e9culaire et Structurale, CENG 85X, Grenoble, France."
        }
      ]
    },
    {
      "given": "Y",
      "family": "Lai",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "D\u00e9partement de Biologie Mol\u00e9culaire et Structurale, CENG 85X, Grenoble, France."
        }
      ]
    },
    {
      "given": "W A",
      "family": "Catterall",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "D\u00e9partement de Biologie Mol\u00e9culaire et Structurale, CENG 85X, Grenoble, France."
        }
      ]
    },
    {
      "given": "M",
      "family": "Ronjat",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "D\u00e9partement de Biologie Mol\u00e9culaire et Structurale, CENG 85X, Grenoble, France."
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1994,
        3,
        15
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.91.6.2270",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T17:49:01Z",
    "timestamp": 1649872141000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.91.6.2270"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1994,
        3,
        15
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1994,
          3,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.91.6.2270"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.91.6.2270",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1994,
        3,
        15
      ]
    ]
  },
  "assertion": [
    {
      "value": "1994-03-15",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}