DOI: 10.1073/pnas.91.4.1381. Trimethylpsoralen induces small deletion mutations inCaenorhabditis elegans.

Trimethylpsoralen induces small deletion mutations inCaenorhabditis elegans.

10.1073/pnas.91.4.1381

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

To examine the mutagenic spectrum of 4,5',8-trimethylpsoralen (TMP) in Caenorhabditis elegans, we isolated mutations in the unc-22 and pal-1 genes following TMP mutagenesis and analyzed them for restriction fragment length polymorphisms by Southern blot. Eleven of 21 unc-22 mutations exhibited restriction fragment length polymorphisms, 8 of which were deletions of between 0.10 and 15 kb in length. Both of two pal-1 mutations were also small deletions within this size range. Comparison of our results with previous studies on mutagenesis by gamma-rays and x-rays suggests that the mutagenic spectrum of TMP may be similar. TMP should be useful in generating mutations that cause complete loss of function of single genes and that are likely to result in allele-specific DNA polymorphisms.

Bibliography

Yandell, M. D., Edgar, L. G., & Wood, W. B. (1994). Trimethylpsoralen induces small deletion mutations inCaenorhabditis elegans. Proceedings of the National Academy of Sciences, 91(4), 1381â1385.

Authors 3

M D Yandell (first)
L G Edgar (additional)
W B Wood (additional)

References 0 Referenced 93

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:03 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 1:48 p.m.)
Indexed	1 year ago (Aug. 23, 2024, 10:39 a.m.)
Issued	31 years, 6 months ago (Feb. 15, 1994)
Published	31 years, 6 months ago (Feb. 15, 1994)
Published Online	31 years, 6 months ago (Feb. 15, 1994)
Published Print	31 years, 6 months ago (Feb. 15, 1994)

Funders 0

None

BibTeX

@article{Yandell_1994, title={Trimethylpsoralen induces small deletion mutations inCaenorhabditis elegans.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.4.1381}, DOI={10.1073/pnas.91.4.1381}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yandell, M D and Edgar, L G and Wood, W B}, year={1994}, month=feb, pages={1381–1385} }

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