DOI: 10.1073/pnas.91.3.836. Atomic force microscopy produces faithful high-resolution images of protein surfaces in an aqueous environment.

Atomic force microscopy produces faithful high-resolution images of protein surfaces in an aqueous environment.

10.1073/pnas.91.3.836

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The atomic force microscope has the potential to monitor structural changes of a biological system in its native environment. To correlate them with the biological function at a molecular level, high lateral and vertical resolution are required. Here we demonstrate that the atomic force microscope is capable of imaging the surface of the hexagonally packed intermediate layer of Deinococcus radiodurans in buffer solution with a lateral resolution of 1 nm and a vertical resolution of 0.1 nm. On average, these topographs differ from those determined by electron microscopy by <0.5 nm.

Bibliography

Karrasch, S., Hegerl, R., Hoh, J. H., Baumeister, W., & Engel, A. (1994). Atomic force microscopy produces faithful high-resolution images of protein surfaces in an aqueous environment. Proceedings of the National Academy of Sciences, 91(3), 836â838.

Authors 5

S Karrasch (first)
R Hegerl (additional)
J H Hoh (additional)
W Baumeister (additional)
A Engel (additional)

References 0 Referenced 130

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 9:03 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 2:02 p.m.)
Indexed	4 months, 1 week ago (April 8, 2025, 7 p.m.)
Issued	31 years, 6 months ago (Feb. 1, 1994)
Published	31 years, 6 months ago (Feb. 1, 1994)
Published Online	31 years, 6 months ago (Feb. 1, 1994)
Published Print	31 years, 6 months ago (Feb. 1, 1994)

Funders 0

None

BibTeX

@article{Karrasch_1994, title={Atomic force microscopy produces faithful high-resolution images of protein surfaces in an aqueous environment.}, volume={91}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.91.3.836}, DOI={10.1073/pnas.91.3.836}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Karrasch, S and Hegerl, R and Hoh, J H and Baumeister, W and Engel, A}, year={1994}, month=feb, pages={836–838} }

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